ArticleAmerican journal of physiology. Cell physiology2010
Effect of chronic contractile activity on mRNA stability in skeletal muscle.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis.Nature communications · 2025Article
- Time-series transcriptomics reveals distinctive mRNA expression dynamics associated with gene ontology specificity and protein expression in skeletal muscle after electrical stimulation-induced resistance exercise.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Review of the current and potential use of biological and molecular methods for the estimation of the postmortem interval in animals and humans.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Review
- A molecular signature defining exercise adaptation with ageing and in vivo partial reprogramming in skeletal muscle.The Journal of physiology · 2023Article
- The transcript interactome of skeletal muscle RNA binding protein motif 3 (RBM3).Physiological reports · 2023Article
- Mitochondria homeostasis: Biology and involvement in hepatic steatosis to NASH.Acta pharmacologica Sinica · 2022Article
- AUF1 gene transfer increases exercise performance and improves skeletal muscle deficit in adult mice.Molecular therapy. Methods & clinical development · 2021Article
- Review
- Skeletal muscle RBM3 expression is associated with extended lifespan in Ames Dwarf and calorie restricted mice.Experimental gerontology · 2021Article
- Fiber-specific and whole-muscle LRP130 expression in rested, exercised, and fasted human skeletal muscle.Pflugers Archiv : European journal of physiology · 2020Article
- The impact of acute and chronic exercise on Nrf2 expression in relation to markers of mitochondrial biogenesis in human skeletal muscle.European journal of applied physiology · 2020Article
- Molecular Basis of Exercise-Induced Skeletal Muscle Mitochondrial Biogenesis: Historical Advances, Current Knowledge, and Future Challenges.Cold Spring Harbor perspectives in medicine · 2018Review
- Low-fat diet, and medium-fat diets containing coconut oil and soybean oil exert different metabolic effects in untrained and treadmill-trained mice.Journal of the International Society of Sports Nutrition · 2018Article
- Protein arginine methyltransferase expression, localization, and activity during disuse-induced skeletal muscle plasticity.American journal of physiology. Cell physiology · 2018Article
- Aging Hallmarks: The Benefits of Physical Exercise.Frontiers in endocrinology · 2018Review
- Diabetes-Induced Dysfunction of Mitochondria and Stem Cells in Skeletal Muscle and the Nervous System.International journal of molecular sciences · 2017Review
- Impact of Aging and Exercise on Mitochondrial Quality Control in Skeletal Muscle.Oxidative medicine and cellular longevity · 2017Review
- Function of specialized regulatory proteins and signaling pathways in exercise-induced muscle mitochondrial biogenesis.Integrative medicine research · 2016Review
- Effect of denervation on the regulation of mitochondrial transcription factor A expression in skeletal muscle.American journal of physiology. Cell physiology · 2015Article
- Exercise attenuates the major hallmarks of aging.Rejuvenation research · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Repeated bouts of exercise promote the biogenesis of mitochondria by multiple steps in the gene expression patterning. The role of mRNA stability in controlling the expression of mitochondrial proteins is relatively unexplored. To induce mitochondrial biogenesis, we chronically stimulated (10 Hz; 3 or 6 h/day) rat muscle for 7 days. Chronic contractile activity (CCA) increased the protein expression of PGC-1alpha, c-myc, and mitochondrial transcription factor A (Tfam) by 1.6-, 1.7- and 2.0-fold, respectively. To determine mRNA stability, we incubated total RNA with cytosolic extracts using an in vitro cell-free system. We found that the intrinsic mRNA half-lives (t(1/2)) were variable within control muscle. Peroxisome proliferator-activated receptor-gamma, coactivator-1alpha (PGC-1alpha) and Tfam mRNAs decayed more rapidly (t(1/2) = 22.7 and 31.4 min) than c-myc mRNA (t(1/2) = 99.7 min). Furthermore, CCA resulted in a differential response in degradation kinetics. After CCA, PGC-1alpha and Tfam mRNA half-lives decreased by 48% and 44%, respectively, whereas c-myc mRNA half-life was unchanged. CCA induced an elevation of both the cytosolic RNA-stabilizing human antigen R (HuR) and destabilizing AUF1 (total) by 2.4- and 1.8-fold, respectively. Increases in the p37(AUF1), p40(AUF1), and p45(AUF1) isoforms were most evident. Thus these data indicate that CCA results in accelerated turnover rates of mRNAs encoding important mitochondrial biogenesis regulators in skeletal muscle. This adaptation is likely beneficial in permitting more rapid phenotypic plasticity in response to subsequent contractile activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.