ArticleJournal of clinical microbiology2010
Development of a loop-mediated isothermal amplification assay for rapid detection of subgroup J avian leukosis virus.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- Article
- Rapid and sensitive visual detection of avian leukosis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow immunochromatographic strip assay.Archives of virology · 2024Article
- Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick.Frontiers in veterinary science · 2024Article
- Simultaneous Detection of Three Subgroups of Avian Leukosis Virus Using the Nanoparticle-Assisted PCR Assay.Viruses · 2023Article
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus.Poultry science · 2022Article
- New rapid detection by using a constant temperature method for avian leukosis viruses.Frontiers in microbiology · 2022Article
- Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research.Animals : an open access journal from MDPI · 2021Review
- Rapid detection of avian leukosis virus subgroup J by cross-priming amplification.Scientific reports · 2021Article
- Colorimetric biosensors for point-of-care virus detections.Materials science for energy technologies · 2020Review
- Rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay.Poultry science · 2019Article
- A cell line resistant to avian leukosis virus subgroup B infection.Poultry science · 2019Article
- Development and application of a SYBR green real-time PCR for detection of the emerging avian leukosis virus subgroup K.Poultry science · 2018Article
- The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection.Archives of virology · 2018Article
- Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostic Device.Sensors and actuators. B, Chemical · 2016Article
- Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification.Virology journal · 2015Article
- Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B.Virology journal · 2015Article
- Rapid detection of hepatitis B virus in blood plasma by a specific and sensitive loop-mediated isothermal amplification assay.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Development and application of a multiplex PCR method for rapid differential detection of subgroup A, B, and J avian leukosis viruses.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infection of breeder flocks in China with subgroup J avian leukosis virus (ALV-J) has increased recently. In this study, we have developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of ALV-J from culture isolates and clinical samples. The ALV-J-specific LAMP assay efficiently amplified the target gene within 45 min at 63 degrees C using only a simple laboratory water bath. To determine the specificity of the LAMP assay, various subgroup ALVs and other related viruses were detected. A ladder pattern on gel electrophoresis was observed for ALV-J isolates but not for other viruses. To evaluate the sensitivities of the LAMP assay and conventional PCR, the NX0101 isolate plasmid DNA was amplified by them. The detection limit of the LAMP assay was 5 target gene copies/reaction, which was up to 20 times higher than that of conventional PCR. To evaluate the application of the LAMP assay for detection of ALV-J in clinical samples, 49 samples suspected of ALV infection from breeder flocks were tested by the LAMP assay and PCR. Moreover, virus isolation from these samples was also performed using cell culture. The positive-sample ratios were 21/49 (43%) by conventional PCR, 26/49 (53%) by the LAMP assay, and 19/46 (41%) by virus isolation. Additionally, a positive LAMP reaction can be visually ascertained by the observation of turbidity or a color change after addition of SYBR green I dye. Consequently, the LAMP assay is a simple, rapid, and sensitive diagnostic method and can potentially be developed for rapid detection of ALV-J infection in the field.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.