ArticleMolecular biology of the cell2010
Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitation.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 46 citations in OpenAlex.
- IFN-α suppresses GATA3 transcription from a distal exon and promotes H3K27 trimethylation of the CNS-1 enhancer in human Th2 cells.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- Transgenerational Transmission of Epigenetic Marks During Reproduction inThe Yale journal of biology and medicine · 2025Review
- Acetylation at lysine 27 on maternal H3.3 regulates minor zygotic genome activation.Cell reports · 2025Article
- Review
- H3K36 trimethylation-mediated biological functions in cancer.Clinical epigenetics · 2021Review
- Histone variants in skeletal myogenesis.Epigenetics · 2021Review
- Review
- Monitoring of chromatin organization in live cells by FRIC. Effects of the inner nuclear membrane protein Samp1.Nucleic acids research · 2019Article
- A versatile mouse model of epitope-tagged histone H3.3 to study epigenome dynamics.The Journal of biological chemistry · 2019Article
- Global H3.3 dynamic deposition defines its bimodal role in cell fate transition.Nature communications · 2018Article
- PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX.Genome research · 2017Article
- Genetic mosaics and time-lapse imaging identify functions of histone H3.3 residues in mouse oocytes and embryos.Development (Cambridge, England) · 2017Article
- RNase P protein subunit Rpp29 represses histone H3.3 nucleosome deposition.Molecular biology of the cell · 2016Article
- H3K27 Methylation: A Focal Point of Epigenetic Deregulation in Cancer.Advances in cancer research · 2016Review
- Histone H3 Variant Regulates RNA Polymerase II Transcription Termination and Dual Strand Transcription of siRNA Loci in Trypanosoma brucei.PLoS genetics · 2016Article
- Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle.Nucleic acids research · 2015Article
- Point mutations in an epigenetic factor lead to multiple types of bone tumors: role of H3.3 histone variant in bone development and disease.BoneKEy reports · 2015Review
- Arsenic induces polyadenylation of canonical histone mRNA by down-regulating stem-loop-binding protein gene expression.The Journal of biological chemistry · 2014Article
- Chromatin dynamics: H3K4 methylation and H3 variant replacement during development and in cancer.Cellular and molecular life sciences : CMLS · 2014Review
- Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to canonical histones, histone variant H3.3 is incorporated into chromatin in a replication-independent manner. Posttranslational modifications of H3.3 have been identified; however, the epigenetic environment of incorporated H3.3 is unclear. We have investigated the genomic distribution of epitope-tagged H3.3 in relation to histone modifications, DNA methylation, and transcription in mesenchymal stem cells. Quantitative imaging at the nucleus level shows that H3.3, relative to replicative H3.2 or canonical H2B, is enriched in chromatin domains marked by histone modifications of active or potentially active genes. Chromatin immunoprecipitation of epitope-tagged H3.3 and array hybridization identified 1649 H3.3-enriched promoters, a fraction of which is coenriched in H3K4me3 alone or together with H3K27me3, whereas H3K9me3 is excluded, corroborating nucleus-level imaging data. H3.3-enriched promoters are predominantly CpG-rich and preferentially DNA methylated, relative to the proportion of methylated RefSeq promoters in the genome. Most but not all H3.3-enriched promoters are transcriptionally active, and coenrichment of H3.3 with repressive H3K27me3 correlates with an enhanced proportion of expressed genes carrying this mark. H3.3-target genes are enriched in mesodermal differentiation and signaling functions. Our data suggest that in mesenchymal stem cells, H3.3 targets lineage-priming genes with a potential for activation facilitated by H3K4me3 in facultative association with H3K27me3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.