ArticleBMC evolutionary biology2010
Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry.
Article in BMC evolutionary biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- Making MALDI-TOF MS for entomological parameters accessible: A practical guide for in-house library creation.PloS one · 2025Article
- A universal tool for marine metazoan species identification: towards best practices in proteomic fingerprinting.Scientific reports · 2024Article
- Evolution of MALDI-TOF MS Profiles from Lice and Fleas Preserved in Alcohol over Time.Insects · 2023Article
- Detection ofFrontiers in veterinary science · 2022Article
- MALDI-TOF MS: optimization for future uses in entomological surveillance and identification of mosquitoes from New Caledonia.Parasites & vectors · 2020Article
- Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex.Molecules (Basel, Switzerland) · 2020Article
- Characterization of the prohormone complement in Amphiprion and related fish species integrating genome and transcriptome assemblies.PloS one · 2020Article
- Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding.Scientific reports · 2019Article
- Rapid identification of medically important mosquitoes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Parasites & vectors · 2018Article
- Molecular and MALDI-TOF identification of ticks and tick-associated bacteria in Mali.PLoS neglected tropical diseases · 2017Article
- MALDI-TOF MS Profiling-Advances in Species Identification of Pests, Parasites, and Vectors.Frontiers in cellular and infection microbiology · 2017Review
- Morphological, molecular and MALDI-TOF mass spectrometry identification of ixodid tick species collected in Oromia, Ethiopia.Parasitology research · 2016Article
- Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS.PLoS neglected tropical diseases · 2016Article
- Two Major Clades of Bradyrhizobia Dominate Symbiotic Interactions with Pigeonpea in Fields of Côte d'Ivoire.Frontiers in microbiology · 2016Article
- Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems.Parasites & vectors · 2015Article
- Detection of Rickettsia spp in ticks by MALDI-TOF MS.PLoS neglected tropical diseases · 2015Article
- Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular biology techniques for identification of Culicoides (Diptera: ceratopogonidae) biting midges in senegal.Journal of clinical microbiology · 2015Article
- MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.Frontiers in microbiology · 2015Review
- Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling.Parasites & vectors · 2014Article
- Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry.Parasites & vectors · 2014Article
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Authors and funding
5 authors.
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Abstract
backgroundThe use of molecular biology-based methods for species identification and establishing phylogenetic relationships has supplanted traditional methods relying on morphological characteristics. While PCR-based methods are now the commonly accepted gold standards for these types of analysis, relatively high costs, time-consuming assay development or the need for a priori information about species-specific sequences constitute major limitations. In the present study, we explored the possibility to differentiate between 13 different species from the genus Drosophila via a molecular proteomic approach.
resultsAfter establishing a simple protein extraction procedure and performing matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) with intact proteins and peptides, we could show that most of the species investigated reproducibly yielded mass spectra that were adequate for species classification. Furthermore, a dendrogram generated by cluster analysis of total protein patterns agrees reasonably well with established phylogenetic relationships.
conclusionConsidering the intra- and interspecies similarities and differences between spectra obtained for specimens of closely related Drosophila species, we estimate that species typing of insects and possibly other multicellular organisms by intact protein profiling (IPP) can be established successfully for species that diverged from a common ancestor about 3 million years ago.
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