ArticleMolecular microbiology2010
A regulatory RNA required for antitermination of biofilm and capsular polysaccharide operons in Bacillales.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 68 citations in OpenAlex.
- Identification of genetic determinants that promote biofilm growth under heterotrophic conditions inBiofilm · 2026Article
- Ribosomal allostery as a potential regulator of bacterial dormancy.Nature communications · 2026Article
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- Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.PLoS genetics · 2024Article
- Defining Early Steps inbioRxiv : the preprint server for biology · 2023Article
- A variant of guanidine-IV riboswitches exhibits evidence of a distinct ligand specificity.RNA biology · 2023Article
- Electrochemical potential enables dormant spores to integrate environmental signals.Science (New York, N.Y.) · 2022Article
- A Sporulation-Specific sRNA Bvs196 Contributing to the Developing Spore inMicroorganisms · 2022Article
- Conjugation Operons in Gram-Positive Bacteria with and without Antitermination Systems.Microorganisms · 2022Article
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Quantitative mapping of mRNA 3' ends in Pseudomonas aeruginosa reveals a pervasive role for premature 3' end formation in response to azithromycin.PLoS genetics · 2021Article
- A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria.Nucleic acids research · 2021Article
- Encoding Membrane-Potential-Based Memory within a Microbial Community.Cell systems · 2020Article
- Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.Frontiers in microbiology · 2020Review
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model BacteriumFrontiers in molecular biosciences · 2019Review
- Processive Antitermination.Microbiology spectrum · 2018Review
- Signal Percolation within a Bacterial Community.Cell systems · 2018Article
- Challenges of ligand identification for the second wave of orphan riboswitch candidates.RNA biology · 2018Article
- Natural RNA Polymerase Aptamers Regulate Transcription in E. coli.Molecular cell · 2017Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formation of a Bacillus subtilis biofilm community requires an abundant matrix protein, TasA and an exopolysaccharide. The transcriptional regulatory pathways that control synthesis of these structural features are complex and responsive to multiple physiological and population signals. We report herein that an additional layer of co-transcriptional regulation is required for exopolysaccharide (eps) expression. This mechanism is mediated by a novel cis-acting RNA element, coined 'EAR', located between the second and third gene of the eps operon. The presence of the EAR element within the eps operon is required for readthrough of distally located termination signals. We also find that the EAR element promotes readthrough of heterologous termination sites. Based upon these observations, we hypothesize that the EAR element associates with RNA polymerase to promote processive antitermination, a process wherein the transcription elongation complex is altered by accessory factors to become resistant to pausing and termination signals. It is likely that this mechanism is required for eps expression to ensure full synthesis of the unusually long transcript (16 kb). We also identify the EAR element in other species within the order Bacillales, suggesting that a similar mechanism is required for synthesis of biofilm and capsular polysaccharide operons in other microorganisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.