ArticleAnalytical chemistry2010
A photocleavable and mass spectrometry identifiable cross-linker for protein interaction studies.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Multidimensional Cross-Linking and Real-Time Informatics for Multiprotein Interaction Studies.Journal of proteome research · 2024Article
- A protein-protein interaction analysis tool for targeted cross-linking mass spectrometry.Scientific reports · 2023Article
- Proximity labeling and other novel mass spectrometric approaches for spatiotemporal protein dynamics.Expert review of proteomics · 2021Review
- Review
- Open Search Strategy for Inferring the Masses of Cross-Link Adducts on Proteins.Analytical chemistry · 2020Article
- Isobaric Quantitative Protein Interaction Reporter Technology for Comparative Interactome Studies.Analytical chemistry · 2020Article
- Chemical cross-linking with mass spectrometry: a tool for systems structural biology.Current opinion in chemical biology · 2019Review
- Cross-Linking Mass Spectrometry: An Emerging Technology for Interactomics and Structural Biology.Analytical chemistry · 2018Review
- The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes.Frontiers in microbiology · 2018Review
- Protein interaction networks at the host-microbe interface inRoyal Society open science · 2017Article
- XLSearch: a Probabilistic Database Search Algorithm for Identifying Cross-Linked Peptides.Journal of proteome research · 2016Article
- Kojak: efficient analysis of chemically cross-linked protein complexes.Journal of proteome research · 2015Article
- Proteomics-based methods for discovery, quantification, and validation of protein-protein interactions.Analytical chemistry · 2013Review
- Mapping protein surface accessibility via an electron transfer dissociation selectively cleavable hydrazone probe.Molecular & cellular proteomics : MCP · 2012Article
- Cross-linking measurements of the Potato leafroll virus reveal protein interaction topologies required for virion stability, aphid transmission, and virus-plant interactions.Journal of proteome research · 2012Article
- Review
- Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions.Analytical chemistry · 2012Article
- In vivo application of photocleavable protein interaction reporter technology.Journal of proteome research · 2012Article
- Quaternary diamines as mass spectrometry cleavable crosslinkers for protein interactions.Journal of the American Society for Mass Spectrometry · 2012Article
- Cross-linking measurements of in vivo protein complex topologies.Molecular & cellular proteomics : MCP · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In this paper, we present the results of proof-of-concept experiments using a novel photocleavable and mass spectrometry identifiable cross-linker pcPIR (photocleavable protein interaction reporter). pcPIR can be dissociated under UV irradiation either off- or online before the introduction to the mass spectrometers. Photo dissociation of cross-linkers is different from either the gas phase or the chemical cleavage of cross-linkers. Different types of cross-links can be identified using the pcPIR mass relationships, where the mass of cross-linked precursor equals the sum of the masses of the released products and reporter. Since pcPIR is cleaved prior to the entrance to the mass spectrometer, the released peptides are available to be sequenced with routine collision-induced dissociation (CID) MS/MS experiments and database search algorithms. In this report, the pcPIR strategy of identifying the cross-linked peptides with on- and off-line photocleavage coupled with novel targeted data dependent LC-MS/MS is demonstrated with the use of standard peptides, bovine serum albumin (BSA), and human hemoglobin tetramer protein complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.