ArticleMagnetic resonance in medicine2010
Gene expression profiling reveals early cellular responses to intracellular magnetic labeling with superparamagnetic iron oxide nanoparticles.
Article in Magnetic resonance in medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Magnetic Cell Targeting for Cardiovascular Tissue Engineering.Tissue engineering. Part B, Reviews · 2025Review
- Metal-Organic Frameworks: Unlocking New Frontiers in Cardiovascular Diagnosis and Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Imaging-guided precision hyperthermia with magnetic nanoparticles.Nature reviews bioengineering · 2025Article
- Application of Iron Nanoparticle-Based Materials in the Food Industry.Materials (Basel, Switzerland) · 2023Review
- Article
- Iron Oxide Nanoparticles in Mesenchymal Stem Cell Detection and Therapy.Stem cell reviews and reports · 2022Review
- Safety Assessments of Nickel Boride Nanoparticles on the Human Pulmonary Alveolar Cells by Using Cell Viability and Gene Expression Analyses.Biological trace element research · 2021Article
- Synthesis of SPIO Nanoparticles and the Subsequent Applications in Stem Cell Labeling for Parkinson's Disease.Nanoscale research letters · 2021Article
- Potential Toxicity of Iron Oxide Magnetic Nanoparticles: A Review.Molecules (Basel, Switzerland) · 2020Review
- Dextran-coated iron oxide nanoparticle-induced nanotoxicity in neuron cultures.Scientific reports · 2020Article
- Impact of Locally Administered Carboxydextran-Coated Super-Paramagnetic Iron Nanoparticles on Cellular Immune Function.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Clinical Tracking of Cell Transfer and Cell Transplantation: Trials and Tribulations.Radiology · 2018Review
- In Vivo MR Imaging of Dual MRI Reporter Genes and Deltex-1 Gene-modified Human Mesenchymal Stem Cells in the Treatment of Closed Penile Fracture.Molecular imaging and biology · 2018Article
- Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties.Nanoscale research letters · 2018Review
- Magnetic Force-Based Microfluidic Techniques for Cellular and Tissue Bioengineering.Frontiers in bioengineering and biotechnology · 2018Review
- Hydrogel-based scaffolds to support intrathecal stem cell transplantation as a gateway to the spinal cord: clinical needs, biomaterials, and imaging technologies.NPJ Regenerative medicine · 2018Review
- Ten Things You Might Not Know about Iron Oxide Nanoparticles.Radiology · 2017Review
- Real-time MRI for precise and predictable intra-arterial stem cell delivery to the central nervous system.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Ferritin heavy chain as a molecular imaging reporter gene in glioma xenografts.Journal of cancer research and clinical oncology · 2017Article
- Recent Advances inEngineering (Beijing, China) · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
With MRI (stem) cell tracking having entered the clinic, studies on the cellular genomic response toward labeling are warranted. Gene expression profiling was applied to C17.2 neural stem cells following superparamagnetic iron oxide/PLL (poly-L-lysine) labeling over the course of 1 week. Relative to unlabeled cells, less than 1% of genes (49 total) exhibited greater than 2-fold difference in expression in response to superparamagnetic iron oxide/PLL labeling. In particular, transferrin receptor 1 (Tfrc) and heme oxygenase 1 (Hmox1) expression was downregulated early, whereas genes involved in lysosomal function (Sulf1) and detoxification (Clu, Cp, Gstm2, Mgst1) were upregulated at later time points. Relative to cells treated with PLL only, cells labeled with superparamagnetic iron oxide/PLL complexes exhibited differential expression of 1399 genes. Though these differentially expressed genes exhibited altered expression over time, the overall extent was limited. Gene ontology analysis of differentially expressed genes showed that genes encoding zinc-binding proteins are enriched after superparamagnetic iron oxide/PLL labeling relative to PLL only treatment, whereas members of the apoptosis/programmed cell death pathway did not display increased expression. Overexpression of the differentially expressed genes Rnf138 and Abcc4 were confirmed by quantitative real-time polymerase chain reaction. These results demonstrate that, although early reactions responsible for iron homeostasis are induced, overall neural stem cell gene expression remains largely unaltered following superparamagnetic iron oxide/PLL labeling.
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