Evidence map›Paper›PMID 20372926›Full record

ArticleParasitology research2010

PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes.

Mohammad N S Al-Sabi, Peter Deplazes, Pia Webster, Jakob L Willesen, Rebecca K Davidson, Christian M O Kapel

Abstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Parasitology research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
8.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 61 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. First report ofThe Canadian veterinary journal = La revue veterinaire canadienne · 2022
    Article
  8. Simultaneous Exposure toPathogens (Basel, Switzerland) · 2021
    Article
  9. Investigation of the occurrence ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021
    Article
  10. Lungworm infections in harbour porpoises (International journal for parasitology. Parasites and wildlife · 2021
    Article
  11. Article
  12. Intra Vitam Diagnosis of NeglectedPathogens (Basel, Switzerland) · 2020
    Article
  13. Rare Case ofHelminthologia · 2019
    Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Repeated inoculations with the lung and heartworm nematodeInternational journal for parasitology. Parasites and wildlife · 2017
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Mohammad N S Al-SabiDepartment of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. nafi@life.ku.dk
Peter Deplazes
Pia Webster
Jakob L Willesen
Rebecca K Davidson
Christian M O Kapel
University of Copenhagen · DKNorwegian Veterinary Institute · NOUniversity of Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

Indexed as

AngiostrongylusAnimalsDNA, HelminthDNA PrimersDNA, Ribosomal SpacerDog DiseasesDogsEuropeFecesFoxesParasitologyPolymerase Chain ReactionSensitivity and SpecificityStrongylida InfectionsDNA, HelminthDNA PrimersDNA, Ribosomal Spacer

Identifiers

PMID20372926
OpenAlexW1977448087

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.