ArticleThe Journal of toxicological sciences2010
Dose-response assessment of four genotoxic chemicals in a combined mouse and rat micronucleus (MN) and Comet assay protocol.
Article in The Journal of toxicological sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 102 citations in OpenAlex.
- Transferability and Reproducibility of the HepaRG CometChip Assay.Environmental and molecular mutagenesis · 2025Article
- An investigation of the genotoxic potential of a well-characterized yerba mate extract.Toxicology reports · 2024Article
- Genotoxicity study of 2-methoxyethanol and benzalkonium chloride through Comet assay using 3D cultured HepG2 cells.Environmental analysis, health and toxicology · 2022Article
- Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats.Toxics · 2022Article
- Acute Oral Administration of Cerium Oxide Nanoparticles Suppresses Lead Acetate-Induced Genotoxicity, Inflammation, and ROS Generation in Mice Renal and Cardiac Tissues.Biological trace element research · 2022Article
- Metal Accumulation and DNA Damage in Oreochromis niloticus and Clarias gariepinus After Chronic Exposure to Discharges of the Batts Drain: Potential Risk to Human Health.Bulletin of environmental contamination and toxicology · 2022Article
- A 9-aminoacridine derivative induces growth inhibition of Ehrlich ascites carcinoma cells and antinociceptive effect in mice.Frontiers in pharmacology · 2022Article
- Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens.Physiological research · 2020Review
- Retracted Article: The synthesis and biological activity of marine alkaloid derivatives and analogues.RSC advances · 2020Article
- Evaluation of 2-methoxy-4-nitroaniline (MNA) in hypersensitivity, 14-day subacute, reproductive, and genotoxicity studies.Toxicology · 2020Article
- Assessment of metal pollution impacts on Tilapia zillii and Mugil cephalus inhabiting Qaroun and Wadi El-Rayan lakes, Egypt, using integrated biomarkers.Environmental science and pollution research international · 2020Article
- Acrylamide Decreases Cell Viability, and Provides Oxidative Stress, DNA Damage, and Apoptosis in Human Colon Adenocarcinoma Cell Line Caco-2.Molecules (Basel, Switzerland) · 2020Article
- Estimation of genomic instability and mutation induction by graphene oxide nanoparticles in mice liver and brain tissues.Environmental science and pollution research international · 2020Article
- 3Rs friendly study designs facilitate rat liver and blood micronucleus assays and Pig-a gene mutation assessments: Proof-of-concept with 13 reference chemicals.Environmental and molecular mutagenesis · 2019Article
- Evaluation of cytological and genetic effects ofSaudi journal of biological sciences · 2019Article
- The effect of different methods and image analyzers on the results of the in vivo comet assay.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Article
- In vivo pig-a and micronucleus study of the prototypical aneugen vinblastine sulfate.Environmental and molecular mutagenesis · 2018Article
- An Integrated Experimental Design for the Assessment of Multiple Toxicological End Points in Rat Bioassays.Environmental health perspectives · 2017Article
- Aneuploidy: a common and early evidence-based biomarker for carcinogens and reproductive toxicants.Environmental health : a global access science source · 2016Review
- Recent advances in in vivo genotoxicity testing: prediction of carcinogenic potential using comet and micronucleus assay in animal models.Journal of cancer prevention · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The in vivo micronucleus (MN) assay has proven to be an effective measure of genotoxicity potential. However, sampling a single tissue (bone marrow) for a single indicator of genetic damage using the MN assay provides a limited genotoxicity profile. The in vivo alkaline (pH >13) Comet assay, which detects a broad spectrum of DNA damage, can be applied to a variety of rodent tissues following administration of test agents. To determine if the Comet assay is a useful supplement to the in vivo MN assay, a combined test protocol (MN/Comet assay) was conducted in male B6C3F1 mice and F344/N rats using four model genotoxicants: ethyl methanesulfonate (EMS), acrylamide (ACM), cyclophosphamide (CP), and vincristine sulfate (VS). Test compounds were administered on 4 consecutive days at 24-hr intervals (VS was administered to rats for 3 days); animals were euthanized 4 hr after the last administration. All compounds induced significant increases in micronucleated reticulocytes (MN-RET) in the peripheral blood of mice, and all but ACM induced MN-RET in rats. EMS and ACM induced significant increases in DNA damage, measured by the Comet assay, in multiple tissues of mice and rats. CP-induced DNA damage was detected in leukocytes and duodenum cells. VS, a spindle fiber disrupting agent, was negative in the Comet assay. Based on these results, the MN/Comet assay holds promise for providing more comprehensive assessments of potential genotoxicants, and the National Toxicology Program (NTP) is presently using this combined protocol in its overall evaluation of the genotoxicity of substances of public health concern.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.