ArticleClinical cancer research : an official journal of the American Association for Cancer Research2010
Targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- The Suicide Gene System Cytosine Deaminase::Uracylphosphoribosyltransferase/5-fluorocytosine Displays a Strong Cytotoxic Effect on Human Melanoma Cells.Current pharmaceutical biotechnology · 2026Article
- Computational microbiome pharmacology analysis elucidates the anti-cancer potential of vaginal microbes and metabolites.Frontiers in microbiology · 2025Article
- Applied models and molecular characteristics of small cell lung cancer.Pathology oncology research : POR · 2024Review
- Engineering Whole Mammalian Cells for Target-Cell-Specific Invasion/Fusion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Expression of the insulinoma-associated 1 (insm1) gene in Xenopus laevis tadpole retina and brain.Gene expression patterns : GEP · 2016Article
- Effects of Two Common Polymorphisms rs2910164 in miR-146a and rs11614913 in miR-196a2 on Gastric Cancer Susceptibility.Gastroenterology research and practice · 2015Review
- Targeting transcriptional addictions in small cell lung cancer with a covalent CDK7 inhibitor.Cancer cell · 2014Article
- RNA interference targeting human FAK and EGFR suppresses human non-small-cell lung cancer xenograft growth in nude mice.Cancer gene therapy · 2013Article
- Insertion of a nuclear factor kappa B DNA nuclear-targeting sequence potentiates suicide gene therapy efficacy in lung cancer cell lines.Cancer gene therapy · 2012Article
- Therapeutic upregulation of Class A scavenger receptor member 5 inhibits tumor growth and metastasis.Cancer science · 2012Article
- Management of malignant pleural effusion by suicide gene therapy in advanced stage lung cancer: a case series and literature review.Cancer gene therapy · 2012Review
- Suicidal gene therapy in the effective control of primary human hepatocellular carcinoma as monitored by noninvasive bioimaging.Gene therapy · 2012Article
- Safeguarding nonhuman primate iPS cells with suicide genes.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- A simple protocol for preparation of a liposomal vesicle with encapsulated plasmid DNA that mediate high accumulation and reporter gene activity in tumor tissue.Results in pharma sciences · 2011Article
- In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection.International journal of nanomedicine · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeSmall cell lung cancer (SCLC) is a highly malignant cancer for which there is no curable treatment. Novel therapies are therefore in great demand. In the present study we investigated the therapeutic effect of transcriptionally targeted suicide gene therapy for SCLC based on the yeast cytosine deaminase (YCD) gene alone or fused with the yeast uracil phosphoribosyl transferase (YUPRT) gene followed by administration of 5-fluorocytosine (5-FC) prodrug. EXPERIMENTAL
designThe YCD gene or the YCD-YUPRT gene was placed under regulation of the SCLC-specific promoter insulinoma-associated 1 (INSM1). Therapeutic effect was evaluated in vitro in SCLC cell lines and in vivo in SCLC xenografted nude mice using the nonviral nanoparticle DOTAP/cholesterol for transgene delivery.
resultsINSM1-YCD/5-FC and INSM1-YCD-YUPRT/5-FC therapy induced high cytotoxicity in a range of SCLC cell lines. The highest therapeutic effect was obtained from the YCD-YUPRT fusion gene strategy. No cytotoxicity was induced after treatment of cell lines of other origin than SCLC. In addition the INSM1-YCD-YUPRT/5-FC therapy was superior to an established suicide gene system consisting of the herpes simplex virus thymidine kinase (HSVTK) gene and the prodrug ganciclovir. The superior effect was in part due to massive bystander cytotoxicity of YCD-YUPRT-produced toxins. Finally, INSM1-YCD-YUPRT/5-FC therapy induced significant tumor growth delay in SCLC xenografts compared with control-treated xenografts.
conclusionsThe current study is the first to test cytosine deaminase-based suicide gene therapy for SCLC and the first to show an antitumor effect from the delivery of suicide gene therapeutics for SCLC in vivo.
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