ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2010
Zebrafish dead end possesses ATPase activity that is required for primordial germ cell development.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Dead-End protein expression, function, and mutation in cancer: a systematic review.Molecular biology reports · 2025Pooled it
- Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.Stem cell reviews and reports · 2026Review
- Transcriptome of Sterile Testes inGenes · 2025Article
- Article
- Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors.Nature communications · 2023Article
- Neural induction drives body axis formation during embryogenesis, but a neural induction-like process drives tumorigenesis in postnatal animals.Frontiers in cell and developmental biology · 2023Review
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- The solution structure of Dead End bound to AU-rich RNA reveals an unusual mode of tandem RRM-RNA recognition required for mRNA regulation.Nature communications · 2022Article
- Spinyhead Croaker Germ Cells GeneLife (Basel, Switzerland) · 2022Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
- Article
- The Role of DND1 in Cancers.Cancers · 2021Review
- Dead-end (dnd) protein in fish-a review.Fish physiology and biochemistry · 2021Review
- Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.Scientific reports · 2020Article
- Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.Molecular reproduction and development · 2018Article
- Maternal Dead-end 1 promotes translation ofDevelopment (Cambridge, England) · 2017Article
- Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus).Fish physiology and biochemistry · 2015Article
- A controllable on-off strategy for the reproductive containment of fish.Scientific reports · 2015Article
- The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).BioMed research international · 2015Article
- Targeted expression in zebrafish primordial germ cells by Cre/loxP and Gal4/UAS systems.Marine biotechnology (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Zebrafish dead end (dnd) mRNA is specifically expressed in primordial germ cells (PGCs) and is required for PGC migration and survival. Previous studies have shown that zebrafish Dnd functions by protecting the 3'UTRs of nanos1 and TDRD7 from miR-430b-mediated RNA deadenylation. In this work, we demonstrate that zebrafish Dnd protein possesses Mg(2+)-dependent ATPase activity that is required for PGC formation. Michaelis-Menten analysis revealed that the ATPase has a k(cat) of 0.632 +/- 0.036/min under optimal conditions, and mapping studies using Dnd truncates showed that ATPase resides in the last 91 aa of the Dnd C terminus. Internal deletion and point mutagenesis analysis of this region were used to identify key amino acids required for ATPase activity. Rescue experiments conducted by injecting mRNAs encoding the Dnd ATPase mutants into embryos in which the endogenous dnd expression was inhibited demonstrated that the ATPase activity is required for normal zebrafish PGC survival. Real-time PCR analysis showed that the expression of PGC markers nanos1 and TDRD7 but not vasa were down-regulated when dnd mutant proteins lacking ATPase were expressed in the rescued embryos, indicating that the Dnd ATPase is involved in protecting nanos1 and TDRD7 transcripts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.