ArticleThe Journal of biological chemistry2010
The T-loop extension of the tomato protein kinase AvrPto-dependent Pto-interacting protein 3 (Adi3) directs nuclear localization for suppression of plant cell death.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2025Article
- Stroke of luck! Antibody off-target leads to a mechanism for regulation of plant defenses.Plant physiology · 2023Article
- An unexpected role for tomato threonine deaminase 2 in host defense against bacterial infection.Plant physiology · 2023Article
- Early Fruit Development Regulation-Related Genes Concordantly Expressed with TCP Transcription Factors in Tomato (Current issues in molecular biology · 2023Article
- Genome-Wide Identification of the AGC Protein Kinase Gene Family Related to Photosynthesis in Rice (International journal of molecular sciences · 2022Article
- Cargo Recognition and Function of Selective Autophagy Receptors in Plants.International journal of molecular sciences · 2021Review
- The MAF1 Phosphoregulatory Region Controls MAF1 Interaction with the RNA Polymerase III C34 Subunit and Transcriptional Repression in Plants.The Plant cell · 2020Article
- SnRK1 Phosphorylates and Destabilizes WRKY3 to Enhance Barley Immunity to Powdery Mildew.Plant communications · 2020Article
- Screening for potential nuclear substrates for the plant cell death suppressor kinase Adi3 using peptide microarrays.PloS one · 2020Article
- Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato.The Plant cell · 2014Article
- Article
- Two Pdk1 phosphorylation sites on the plant cell death suppressor Adi3 contribute to substrate phosphorylation.Biochimica et biophysica acta · 2013Article
- Role of AGC kinases in plant growth and stress responses.Cellular and molecular life sciences : CMLS · 2012Review
- The β-subunit of the SnRK1 complex is phosphorylated by the plant cell death suppressor Adi3.Plant physiology · 2012Article
- Evolutionary Adaptations of Plant AGC Kinases: From Light Signaling to Cell Polarity Regulation.Frontiers in plant science · 2012Article
- The role of vacuolar processing enzyme (VPE) from Nicotiana benthamiana in the elicitor-triggered hypersensitive response and stomatal closure.Journal of experimental botany · 2010Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In tomato (Solanum lycopersicum), resistance to Pseudomonas syringae pv. tomato is elicited by the interaction of the host Pto kinase with the pathogen effector protein AvrPto, which leads to various immune responses including localized cell death termed the hypersensitive response. The AGC kinase Adi3 functions to suppress host cell death and interacts with Pto only in the presence of AvrPto. The cell death suppression (CDS) activity of Adi3 requires phosphorylation by 3-phosphoinositide-dependent protein kinase 1 (Pdk1) and loss of Adi3 function is associated with the hypersensitive response cell death initiated by the Pto/AvrPto interaction. Here we studied the relationship between Adi3 cellular localization and its CDS activity. Adi3 is a nuclear-localized protein, and this localization is dictated by a nuclear localization signal found in the Adi3 T-loop extension, an approximately 80 amino acid insertion into the T-loop, or activation loop, which is phosphorylated for kinase activation. Nuclear localization of Adi3 is required for its CDS activity and loss of nuclear localization causes elimination of Adi3 CDS activity and induction of cell death. This nuclear localization of Adi3 is dependent on Ser-539 phosphorylation by Pdk1 and non-nuclear Adi3 is found in punctate structures throughout the cell. Our data support a model in which Pdk1 phosphorylation of Adi3 directs nuclear localization for CDS and that disruption of Adi3 nuclear localization may be a mechanism for induction of cell death such as that during the Pto/AvrPto interaction.
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