ArticleBiophysical journal2010
Balanced electrostatic and structural forces guide the large conformational change associated with maturation of T = 4 virus.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Unraveling the maturation pathway of a eukaryotic virus through cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Fast viral dynamics revealed by microsecond time-resolved cryo-EM.Nature communications · 2023Article
- Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization.Archives of virology · 2021Review
- Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.Communications biology · 2021Article
- pH stability and disassembly mechanism of wild-type simian virus 40.Soft matter · 2020Article
- Studying viruses using solution X-ray scattering.Biophysical reviews · 2020Review
- Article
- A method for efficient Bayesian optimization of self-assembly systems from scattering data.BMC systems biology · 2018Article
- Emerging applications of small angle solution scattering in structural biology.Protein science : a publication of the Protein Society · 2015Review
- Review
- Article
- Assembly and maturation of a T = 4 quasi-equivalent virus is guided by electrostatic and mechanical forces.Viruses · 2014Review
- Virus assembly and maturation: auto-regulation through allosteric molecular switches.Journal of molecular biology · 2013Review
- Confessions of an icosahedral virus crystallographer.Microscopy (Oxford, England) · 2013Review
- Go hybrid: EM, crystallography, and beyond.Current opinion in structural biology · 2012Review
- Dissecting quasi-equivalence in nonenveloped viruses: membrane disruption is promoted by lytic peptides released from subunit pentamers, not hexamers.Journal of virology · 2012Article
- Integrative structural modeling with small angle X-ray scattering profiles.BMC structural biology · 2012Review
- Virus maturation.Annual review of biophysics · 2012Review
- Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Nudaurelia capensis omega virus has a well-characterized T = 4 capsid that undergoes a pH-dependent large conformational changes (LCC) and associated auto-catalytic cleavage of the subunit. We examined previously the particle size at different pH values and showed that maturation occurred at pH 5.5. We now characterized the LCC with time-resolved small-angle x-ray scattering and showed that there were three kinetic stages initiated with an incremental drop in pH: 1), a rapid (<10 ms) collapse to an incrementally smaller particle; 2), a continuous size reduction over the next 5 s; and 3), a smaller final transition occurring in 2-3 min. Equilibrium measurements similar to those reported previously, but now more precise, showed that the particle dimension between pH 5.5 and 5 requires the autocatalytic cleavage to achieve its final compact size. A balance of electrostatic and structural forces shapes the energy landscape of the LCC with the latter requiring annealing of portions of the subunit. Equilibrium experiments showed that many intermediate states could be populated with a homogeneous ensemble of particles by carefully controlling the pH. A titration curve for the LCC was generated that showed that the virtual pK(a) (i.e., the composite of all titratable residues that contribute to the LCC) is 5.8.
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