ArticleBiophysical journal2010
Direct visualization of large and protein-free hemifusion diaphragms.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Using multiscale molecular dynamics simulations to explore the fusion machinery underlying neurotransmitter release.Quarterly reviews of biophysics · 2025Review
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion.Scientific reports · 2023Article
- Three membrane fusion pore families determine the pathway to pore dilation.Biophysical journal · 2023Article
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- A hemifused complex is the hub in a network of pathways to membrane fusion.Biophysical journal · 2023Article
- Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids.ACS synthetic biology · 2022Article
- Mechanism of Membrane Fusion: Interplay of Lipid and Peptide.The Journal of membrane biology · 2022Review
- How proteins open fusion pores: insights from molecular simulations.European biophysics journal : EBJ · 2021Review
- Sequential Water and Headgroup Merger: Membrane Poration Paths and Energetics from MD Simulations.Biophysical journal · 2020Article
- Asymmetric Bilayers by Hemifusion: Method and Leaflet Behaviors.Biophysical journal · 2019Article
- Glycan-decorated protocells: novel features for rebuilding cellular processes.Interface focus · 2019Review
- Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.Biophysical journal · 2019Article
- Article
- Effective in Vivo Targeting of Influenza Virus through a Cell-Penetrating/Fusion Inhibitor Tandem Peptide Anchored to the Plasma Membrane.Bioconjugate chemistry · 2018Article
- SNARE-mediated membrane fusion arrests at pore expansion to regulate the volume of an organelle.The EMBO journal · 2018Article
- Sculpting and fusing biomimetic vesicle networks using optical tweezers.Nature communications · 2018Article
- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- Regulation of Exocytotic Fusion Pores by SNARE Protein Transmembrane Domains.Frontiers in molecular neuroscience · 2017Review
- Visualization of SNARE-Mediated Hemifusion between Giant Unilamellar Vesicles Arrested by Myricetin.Frontiers in molecular neuroscience · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusion of cellular membranes is a ubiquitous biological process requiring remodeling of two phospholipid bilayers. We believe it is very likely that merging of membranes proceeds via similar sequential intermediates. Contacting membranes form a stalk between the proximal leaflets that expands radially into an hemifusion diaphragm (HD) and subsequently open to a fusion pore. Although considered to be a key intermediate in fusion, direct experimental verification of this structure is difficult due to its transient nature. Using confocal fluorescence microscopy we have investigated the fusion of giant unilamellar vesicles (GUVs) containing phosphatidylserine and fluorescent virus derived transmembrane peptides or membrane proteins in the presence of divalent cations. Time-resolved imaging revealed that fusion was preceded by displacement of peptides and fluorescent lipid analogs from the GUV-GUV adhesion region. A detailed analysis of this area being several mum in size revealed that peptides were completely sequestered as expected for an HD. Lateral distribution of lipid analogs was consistent with formation of an HD but not with the presence of two adherent bilayers. Formation and size of the HD were dependent on lipid composition and peptide concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.