ArticleThe Biochemical journal2010
A novel HPLC-based approach makes possible the spatial characterization of cellular PtdIns5P and other phosphoinositides.
Article in The Biochemical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 124 citations in OpenAlex.
- Mechanisms and Advances in Plant Lipid Regulatory Responses Under Biotic and Abiotic Stress.Genes · 2026Review
- Synthesis and characterization of a novel phosphatidylinositol 5-phosphate (PI(5)P) photoaffinity probe.RSC chemical biology · 2026Article
- Subcellular Cartography of the Phosphoinositide Multiverse.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2026Review
- PI5P4K inhibitors: promising opportunities and challenges.The FEBS journal · 2025Review
- Multimodal action of KRP203 on phosphoinositide kinases in vitro and in cells.Biochemical and biophysical research communications · 2023Article
- Review
- A novel homeostatic mechanism tunes PI(4,5)P2-dependent signaling at the plasma membrane.Journal of cell science · 2023Article
- PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.Science advances · 2023Article
- 25 Years of PI5P.Frontiers in cell and developmental biology · 2023Review
- PI(4,5)P2: signaling the plasma membrane.The Biochemical journal · 2022Article
- Molecular basis of hUHRF1 allosteric activation for synergistic histone modification binding by PI5P.Science advances · 2022Article
- The distribution of phosphatidylinositol 4,5-bisphosphate in the budding yeast plasma membrane.Histochemistry and cell biology · 2021Article
- Pharmacological inhibition of PI5P4Kα/β disrupts cell energy metabolism and selectively kills p53-null tumor cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phosphoinositides signaling modulates microglial actin remodeling and phagocytosis in Alzheimer's disease.Cell communication and signaling : CCS · 2021Review
- Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Crucial Players for Inter-Organelle Communication: PI5P4Ks and Their Lipid Product PI-4,5-PFrontiers in cell and developmental biology · 2021Article
- Lipid transfer proteins and instructive regulation of lipid kinase activities: Implications for inositol lipid signaling and disease.Advances in biological regulation · 2020Review
- Novel defect in phosphatidylinositol 4-kinase type 2-alpha (PI4K2A) at the membrane-enzyme interface is associated with metabolic cutis laxa.Journal of inherited metabolic disease · 2020Article
- Defining the subcellular distribution and metabolic channeling of phosphatidylinositol.The Journal of cell biology · 2020Article
- The nuclear phosphoinositide response to stress.Cell cycle (Georgetown, Tex.) · 2020Review
16 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
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Abstract
PtdIns5P was discovered in 1997 [Rameh, Tolias, Duckworth and Cantley (1997) Nature 390, 192-196], but still very little is known about its regulation and function. Hitherto, studies of PtdIns5P regulation have been hindered by the inability to measure cellular PtdIns5P using conventional HPLC, owing to poor separation from PtdIns4P. In the present paper we describe a new HPLC method for resolving PtdIns5P from PtdIns4P, which makes possible accurate measurements of basal and inducible levels of cellular PtdIns5P in the context of other phosphoinositides. Using this new method, we found that PtdIns5P is constitutively present in all cells examined (epithelial cells, fibroblasts and myoblasts, among others) at levels typically 1-2% of PtdIns4P levels. In the beta-pancreatic cell line BTC6, which is specialized in insulin secretion, PtdIns5P levels were higher than in most cells (2.5-4% of PtdIns4P). Using subcellular fractionation, we found that the majority of the basal PtdIns5P is present in the plasma membrane, but it is also enriched in intracellular membrane compartments, especially in SER (smooth endoplasmic reticulum) and/or Golgi, where high levels of PtdIns3P were also detected. Unlike PtdIns3P, PtdIns5P was also found in fractions containing very-low-density vesicles. Knockdown of PIP4K (PtdIns5P 4-kinase) leads to accumulation of PtdIns5P in light fractions and fractions enriched in SER/Golgi, whereas treatment with Brefeldin A results in a subtle, but reproducible, change in PtdIns5P distribution. These results indicate that basal PtdIns5P and the PtdIns5P pathway for PtdIns(4,5)P(2) synthesis may play a role in Golgi-mediated vesicle trafficking.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.