ArticleBiological chemistry2010
Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases.
Article in Biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- The Histamine Four Receptor Regulates LFA-1 But Not Mac-1 -Dependent Neutrophil Adhesion and Inflammatory Functions.Inflammation · 2026Article
- Basic, translational and clinical aspects of bronchiectasis in adults.European respiratory review : an official journal of the European Respiratory Society · 2023Article
- Elafin and its precursor trappin-2: What is their therapeutic potential for intestinal diseases?British journal of pharmacology · 2023Review
- Specific interactions between the alkaline protease of P. aeruginosa and its natural peptide inhibitor: ab initio molecular simulations.Journal of molecular modeling · 2021Article
- Impact of Bacterial Toxins in the Lungs.Toxins · 2020Review
- Impaired Airway Epithelial Barrier Integrity in Response toFrontiers in immunology · 2020Article
- Characterisation of the Major Extracellular Proteases ofPathogens (Basel, Switzerland) · 2019Article
- Thrombospondin-1 protects against pathogen-induced lung injury by limiting extracellular matrix proteolysis.JCI insight · 2018Article
- The Role of Serine Proteases and Antiproteases in the Cystic Fibrosis Lung.Mediators of inflammation · 2015Review
- A functional variant of elafin with improved anti-inflammatory activity for pulmonary inflammation.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- MicroRNA Dysregulation in Cystic Fibrosis.Mediators of inflammation · 2015Review
- Functional characterization of Anopheles matrix metalloprotease 1 reveals its agonistic role during sporogonic development of malaria parasites.Infection and immunity · 2014Article
- Protease-armed bacteria in the skin.Cell and tissue research · 2013Review
- Proteases, cystic fibrosis and the epithelial sodium channel (ENaC).Cell and tissue research · 2013Review
- Airway inflammatory markers in individuals with cystic fibrosis and non-cystic fibrosis bronchiectasis.Journal of inflammation research · 2013Article
- Activation of the epithelial sodium channel (ENaC) by the alkaline protease from Pseudomonas aeruginosa.The Journal of biological chemistry · 2012Article
- Calcium-induced folding and stabilization of the Pseudomonas aeruginosa alkaline protease.The Journal of biological chemistry · 2012Article
- Periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid.Molecular oral microbiology · 2012Article
- Trappin-2/elafin modulate innate immune responses of human endometrial epithelial cells to PolyI:C.PloS one · 2012Article
- Pulmonary proteases in the cystic fibrosis lung induce interleukin 8 expression from bronchial epithelial cells via a heme/meprin/epidermal growth factor receptor/Toll-like receptor pathway.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
Abstract
Elafin is a 6-kDa innate immune protein present at several epithelial surfaces including the pulmonary epithelium. It is a canonical protease inhibitor of two neutrophil serine proteases [neutrophil elastase (NE) and proteinase 3] with the capacity to covalently bind extracellular matrix proteins by transglutamination. In addition to these properties, elafin also possesses antimicrobial and immunomodulatory activities. The aim of the present study was to investigate the effect of Pseudomonas aeruginosa proteases on elafin function. We found that P. aeruginosa PAO1-conditioned medium and two purified Pseudomonas metalloproteases, pseudolysin (elastase) and aeruginolysin (alkaline protease), are able to cleave recombinant elafin. Pseudolysin was shown to inactivate the anti-NE activity of elafin by cleaving its protease-binding loop. Interestingly, antibacterial properties of elafin against PAO1 were found to be unaffected after pseudolysin treatment. In contrast to pseudolysin, aeruginolysin failed to inactivate the inhibitory properties of elafin against NE. Aeruginolysin cleaves elafin at the amino-terminal Lys6-Gly7 peptide bond, resulting in a decreased ability to covalently bind purified fibronectin following transglutaminase activity. In conclusion, this study provides evidence that elafin is susceptible to proteolytic cleavage at alternative sites by P. aeruginosa metalloproteinases, which can affect different biological functions of elafin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.