ArticlePlant cell reports2010
Putative storage root specific promoters from cassava and yam: cloning and evaluation in transgenic carrots as a model system.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Generating colorful carrot germplasm through metabolic engineering of betalains pigments.Horticulture research · 2023Article
- Identification of essential element determining fruit-specific transcriptional activity in the tomato HISTIDINE DECARBOXYLASE A gene promoter.Plant cell reports · 2022Article
- A promoter toolbox for tissue-specific expression supporting translational research in cassava (Frontiers in plant science · 2022Article
- Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects.Planta · 2021Review
- A Roadmap to Modulated Anthocyanin Compositions in Carrots.Plants (Basel, Switzerland) · 2021Review
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter.Plant cell reports · 2019Article
- Carrot cells: a pioneering platform for biopharmaceuticals production.Molecular biotechnology · 2015Review
- Carotene Hydroxylase Activity Determines the Levels of Both α-Carotene and Total Carotenoids in Orange Carrots.The Plant cell · 2014Article
- A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière).International journal of molecular sciences · 2013Article
- Cloning and characterization of a tuberous root-specific promoter from cassava (Manihot esculenta Crantz).Planta · 2012Article
- A sweetpotato SRD1 promoter confers strong root-, taproot-, and tuber-specific expression in Arabidopsis, carrot, and potato.Transgenic research · 2012Article
- Characterization of phytoene synthases from cassava and their involvement in abiotic stress-mediated responses.Planta · 2010Article
- Provitamin A accumulation in cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene.The Plant cell · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A prerequisite for biotechnological improvements of storage roots is the availability of tissue-specific promoters enabling high expression of transgenes. In this work, we cloned two genomic fragments, pMe1 and pDJ3S, controlling the expression of a gene with unknown function from cassava (Manihot esculenta) and of the storage protein dioscorin 3 small subunit gene from yam (Dioscorea japonica), respectively. Using beta-glucuronidase as a reporter, the activities of pMe1 and pDJ3S were evaluated in independent transgenic carrot lines and compared to the constitutive CaMV35S and the previously described cassava p15 promoters. Activities of pMe1 and pDJ3S in storage roots were assessed using quantitative GUS assays that showed pDJ3S as the most active one. To determine organ specificities, uidA transcript levels in leaves, stems and roots were measured by real-time RT-PCR analyses showing highest storage root specificity for pDJ3S. Root cross sections revealed that pMe1 was highly active in secondary xylem. In contrast, pDJ3S was active in all root tissues except for the central xylem. The expression patterns caused by the cassava p15 promoter in carrot storage roots were consistent with its previously described activities for the original storage organ. Our data demonstrate that the pDJ3S and, to a lesser extent, the pMe1 regulatory sequences represent feasible candidates to drive high and preferential expression of genes in carrot storage roots.
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