ArticlePloS one2010
Characterization of trophoblast and extraembryonic endoderm cell lineages derived from rat preimplantation embryos.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Derivation of porcine extraembryonic endoderm-like cells from blastocysts.Cell proliferation · 2020Article
- From engineering to editing the rat genome.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- A focused microarray for screening rat embryonic stem cell lines.Stem cells and development · 2013Article
- Robust self-renewal of rat embryonic stem cells requires fine-tuning of glycogen synthase kinase-3 inhibition.Stem cell reports · 2013Article
- Derivation and characterization of embryonic stem cells lines derived from transgenic Fischer 344 and Dark Agouti rats.Stem cells and development · 2012Article
- Rat placentation: an experimental model for investigating the hemochorial maternal-fetal interface.Placenta · 2012Review
- Cellular diversity within embryonic stem cells: pluripotent clonal sublines show distinct differentiation potential.Journal of cellular and molecular medicine · 2012Article
- Troika of the mouse blastocyst: lineage segregation and stem cells.Current stem cell research & therapy · 2012Review
- FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage.Developmental biology · 2011Article
- Article
- Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.PloS one · 2011Article
- Phosphatidylinositol 3 kinase modulation of trophoblast cell differentiation.BMC developmental biology · 2010Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious attempts to isolate pluripotent cell lines from rat preimplantation embryo in mouse embryonic stem (ES) cell culture conditions (serum and LIF) were unsuccessful, however the resulting cells exhibited the expression of such traditional pluripotency markers as SSEA-1 and alkaline phosphatase. We addressed the question, which kind of cell lineages are produced from rat preimplantation embryo under "classical" mouse ES conditions.
resultsWe characterized two cell lines (C5 and B10) which were obtained from rat blastocysts in medium with serum and LIF. In the B10 cell line we found the expression of genes known to be expressed in trophoblast, Cdx-2, cytokeratin-7, and Hand-1. Also, B10 cells invaded the trophectodermal layer upon injection into rat blastocysts. In contrast to mouse Trophoblast Stem (TS) cells proliferation of B10 cells occurred independently of FGF4. Cells of the C5 line expressed traditional markers of extraembryonic-endoderm (XEN) cells, in particular, GATA-4, but also the pluripotency markers SSEA-1 and Oct-4. C5 cell proliferation exhibited dependence on LIF, which is not known to be required by mouse XEN cells.
conclusionsOur results confirm and extend previous findings about differences between blastocyst-derived cell lines of rat and mice. Our data show, that the B10 cell line represents a population of FGF4-independent rat TS-like cells. C5 cells show features that have recently become known as characteristic of rat XEN cells. Early passages of C5 and B10 cells contained both, TS and XEN cells. We speculate, that mechanisms maintaining self-renewal of cell lineages in rat preimplantation embryo and their in vitro counterparts, including ES, TS and XEN cells are different than in respective mouse lineages.
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