ArticleACS chemical neuroscience2010
Only a Fraction of Quantal Content is Released During Exocytosis as Revealed by Electrochemical Cytometry of Secretory Vesicles.
Article in ACS chemical neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 160 citations in OpenAlex.
- Computational modelling identifies key determinants of subregion-specific dopamine dynamics in the striatum.eLife · 2026Article
- Aluminum Exposure Impairs Electrochemically Measured Vesicular Storage and Exocytosis via Iron Homeostasis Dysregulation.Angewandte Chemie (International ed. in English) · 2025Article
- Electrochemical Droplet Sculpturing of Short Carbon Fiber Nanotip Electrodes for Neurotransmitter Detection.ACS electrochemistry · 2025Article
- Exploiting Microelectrode Geometry for Comprehensive Detection of Individual Exocytosis Events at Single Cells.ACS sensors · 2023Article
- Neurotransmitter Readily Escapes Detection at the Opposing Microelectrode Surface in Typical Amperometric Measurements of Exocytosis at Single Cells.Analytical chemistry · 2022Article
- Single-Vesicle Electrochemistry Following Repetitive Stimulation Reveals a Mechanism for Plasticity Changes with Iron Deficiency.Angewandte Chemie (International ed. in English) · 2022Article
- Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells.ACS nano · 2022Article
- Quantifying Intracellular Single Vesicular Catecholamine Concentration with Open Carbon Nanopipettes to Unveil the Effect of L-DOPA on Vesicular Structure.Angewandte Chemie (International ed. in English) · 2022Article
- Nano-analysis Reveals High Fraction of Serotonin Release during Exocytosis from a Gut Epithelium Model Cell.Angewandte Chemie (International ed. in English) · 2021Article
- Simultaneous detection of vesicular content and exocytotic release with two electrodes in and at a single cell.Chemical science · 2021Article
- Nanoscale Amperometry Reveals that Only a Fraction of Vesicular Serotonin Content is Released During Exocytosis from Beta Cells.Angewandte Chemie (International ed. in English) · 2021Article
- Electrochemical Quantification of Neurotransmitters in Single Live Cell Vesicles Shows Exocytosis is Predominantly Partial.Chembiochem : a European journal of chemical biology · 2021Review
- Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal-oxide-semiconductor electrode array.Nature communications · 2021Article
- A multimodal electrochemical approach to measure the effect of zinc on vesicular content and exocytosis in a single cell model of ischemia.QRB discovery · 2021Article
- Mass Spectrometric Imaging of Plasma Membrane Lipid Alteration Correlated with Amperometrically Measured Activity-Dependent Plasticity in Exocytosis.International journal of molecular sciences · 2020Article
- Real-Time Measurement of Stimulated Dopamine Release in Compartments of the AdultAnalytical chemistry · 2020Article
- Recent Progress in Quantitatively Monitoring Vesicular Neurotransmitter Release and Storage With Micro/Nanoelectrodes.Frontiers in chemistry · 2020Review
- Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Chromaffin Cells of the Adrenal Medulla: Physiology, Pharmacology, and Disease.Comprehensive Physiology · 2019Article
- Electrochemistry at the Synapse.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
The primary method for neuronal communication involves the release of chemical messengers that are packaged intracellularly in vesicles. Although experiments measuring release at single cells have classically been thought to assess the entire content of vesicles, there is evidence in the literature that suggests that the total transmitter stored in vesicles is not expelled during exocytosis. In this work, we introduce a novel technology using a microfluidic-based platform to electrochemically probe individual PC12 cell vesicles isolated from the cell environment. We measure the total vesicular content using methodology that circumvents the biophysical processes of the cell associated with exocytosis. Direct comparisons of amperometric data from release experiments at single PC12 cells versus our cell-free model reveal that on average vesicles release only 40% of their total transmitter load. The data support the intriguing hypothesis that the average vesicle does not open all the way during the normal exocytosis process, resulting in incomplete distention of the vesicular contents. In addition, we have shown that vesicular catecholamine levels can be altered with pharmacological manipulation and variances observed from these treatments can be resolved at the single vesicle level in a high-throughput manner, a process that we have termed electrochemical cytometry. Upon establishing that release in exocytotic processes proceeds in an incomplete manner, electrochemical data quantified from both single cell release experiments and electrochemical cytometry of vesicles were related to vesicular volume from electron microscopy measurements to investigate the location of intravesicular catecholamine stores retained post-fusion.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.