ArticleProceedings of the National Academy of Sciences of the United States of America2010
Dynamic sorting of lipids and proteins in membrane tubes with a moving phase boundary.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Programming Nonlinear Interfacial Mechanics of Synthetic Cells: Lipid Geometry and DNA Nanostructures.Small science · 2026Article
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Curvature Footprints of Transmembrane Proteins in Simulations with the Martini Force Field.The journal of physical chemistry. B · 2024Article
- Membrane nanotubes transform into double-membrane sheets at condensate droplets.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Nanoscale membrane curvature sorts lipid phases and alters lipid diffusion.Biophysical journal · 2023Article
- Review
- Molecular Shape Solution for Mesoscopic Remodeling of Cellular Membranes.Annual review of biophysics · 2022Review
- Superelasticity of Plasma- and Synthetic Membranes Resulting from Coupling of Membrane Asymmetry, Curvature, and Lipid Sorting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Lipid-Composition-Mediated Forces Can Stabilize Tubular Assemblies of I-BAR Proteins.Biophysical journal · 2021Article
- Review of PIP2 in Cellular Signaling, Functions and Diseases.International journal of molecular sciences · 2020Review
- Geometric pinning and antimixing in scaffolded lipid vesicles.Nature communications · 2020Article
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Lipid Bilayer Thickness Measured by Quantitative DIC Reveals Phase Transitions and Effects of Substrate Hydrophilicity.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- PIP2 Reshapes Membranes through Asymmetric Desorption.Biophysical journal · 2019Article
- Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers.Scientific reports · 2019Article
- Mechanics of the Formation, Interaction, and Evolution of Membrane Tubular Structures.Biophysical journal · 2019Article
- Article
- Giant Vesicles Encapsulating Aqueous Two-Phase Systems: From Phase Diagrams to Membrane Shape Transformations.Frontiers in chemistry · 2019Review
- Molecular mechanisms of force production in clathrin-mediated endocytosis.FEBS letters · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular organelle membranes maintain their integrity, global shape, and composition despite vigorous exchange among compartments of lipids and proteins during trafficking and signaling. Organelle homeostasis involves dynamic molecular sorting mechanisms that are far from being understood. In contrast, equilibrium thermodynamics of membrane mixing and sorting, particularly the phase behavior of binary and ternary model membrane mixtures and its coupling to membrane mechanics, is relatively well characterized. Elucidating the continuous turnover of live cell membranes, however, calls for experimental and theoretical membrane models enabling manipulation and investigation of directional mass transport. Here we introduce the phenomenon of curvature-induced domain nucleation and growth in membrane mixtures with fluid phase coexistence. Membrane domains were consistently observed to nucleate precisely at the junction between a strongly curved cylindrical (tube) membrane and a pipette-aspirated giant unilamellar vesicle. This experimental geometry mimics intracellular sorting compartments, because they often show tubular-vesicular membrane regions. Nucleated domains at tube necks were observed to present diffusion barriers to the transport of lipids and proteins. We find that curvature-nucleated domains grow with characteristic parabolic time dependence that is strongly curvature-dependent. We derive an analytical model that reflects the observed growth dynamics. Numerically calculated membrane shapes furthermore allow us to elucidate mechanical details underlying curvature-dependent directed lipid transport. Our observations suggest a novel dynamic membrane sorting principle that may contribute to intracellular protein and lipid sorting and trafficking.
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