ArticleProceedings of the National Academy of Sciences of the United States of America2010
Legionella pneumophila 6S RNA optimizes intracellular multiplication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 89 citations in OpenAlex.
- Bacterial small regulatory RNAs.Nature reviews. Microbiology · 2026Review
- Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiontMicrobial genomics · 2025Article
- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria.Nucleic acids research · 2024Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- Prokaryotic ncRNAs: Master regulators of gene expression.Current research in pharmacology and drug discovery · 2022Article
- Ms1 RNA Interacts With the RNA Polymerase Core inFrontiers in microbiology · 2022Article
- The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae.Applied and environmental microbiology · 2021Article
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms.The ISME journal · 2021Article
- Similarities and differences between 6S RNAs from Bradyrhizobium japonicum and Sinorhizobium meliloti.Journal of microbiology (Seoul, Korea) · 2020Article
- Riboregulation in Nitrogen-Fixing Endosymbiotic Bacteria.Microorganisms · 2020Review
- Characterization of 6S RNA in the Lyme disease spirochete.Molecular microbiology · 2020Article
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- Bacterial Small RNAs in Mixed Regulatory Networks.Microbiology spectrum · 2018Review
- 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.RNA biology · 2017Article
- Legionella pneumophila OxyR Is a Redundant Transcriptional Regulator That Contributes to Expression Control of the Two-Component CpxRA System.Journal of bacteriology · 2017Article
- Small Non-Coding RNAs: New Insights in Modulation of Host Immune Response by Intracellular Bacterial Pathogens.Frontiers in immunology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Legionella pneumophila is a Gram-negative opportunistic human pathogen that infects and multiplies in a broad range of phagocytic protozoan and mammalian phagocytes. Based on the observation that small regulatory RNAs (sRNAs) play an important role in controlling virulence-related genes in several pathogenic bacteria, we attempted to identify sRNAs expressed by L. pneumophila. We used computational prediction followed by experimental verification to identify and characterize sRNAs encoded in the L. pneumophila genome. A 50-mer probe microarray was constructed to test the expression of predicted sRNAs in bacteria grown under a variety of conditions. This strategy successfully identified 22 expressed RNAs, out of which 6 were confirmed by northern blot and RACE. One of the identified sRNAs is highly expressed in postexponential phase, and computational prediction of its secondary structure reveals a striking similarity to the structure of 6S RNA, a widely distributed prokaryotic sRNA, known to regulate the activity of sigma(70)-containing RNA polymerase. A 70-mer probe microarray was used to identify genes affected by L. pneumophila 6S RNA in stationary phase. The 6S RNA positively regulates expression of genes encoding type IVB secretion system effectors, stress response genes such as groES and recA, as well as many genes involved in acquisition of nutrients and genes with unknown or hypothetical functions. Deletion of 6S RNA significantly reduced L. pneumophila intracellular multiplication in both protist and mammalian host cells, but had no detectable effect on growth in rich media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.