ArticleGenome research2010
Genomic, proteomic, and transcriptomic analysis of virulent and avirulent Rickettsia prowazekii reveals its adaptive mutation capabilities.
Article in Genome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Genomic evolution and adaptation of arthropod-associated Rickettsia.Scientific reports · 2022Pooled it
- Stomach as the target organ of Rickettsia heilongjiangensis infection in C57BL/6 mice identified by click chemistry.Communications biology · 2024Article
- Article
- Genetic typing of isolates of Rickettsia typhi.PLoS neglected tropical diseases · 2022Article
- New approaches in the systematics of rickettsiae.New microbes and new infections · 2018Article
- Comparative Transcriptome Profiling of Virulent and AttenuatedFrontiers in cellular and infection microbiology · 2018Article
- Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever GroupFrontiers in microbiology · 2017Article
- Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells.Frontiers in cellular and infection microbiology · 2016Article
- Economic Game Theory to Model the Attenuation of Virulence of an Obligate Intracellular Bacterium.Frontiers in cellular and infection microbiology · 2016Article
- Origin and Evolution of Rickettsial Plasmids.PloS one · 2016Article
- Rickettsia buchneri sp. nov., a rickettsial endosymbiont of the blacklegged tick Ixodes scapularis.International journal of systematic and evolutionary microbiology · 2015Article
- Article
- An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.PLoS pathogens · 2015Article
- Post-translational modification of LipL32 during Leptospira interrogans infection.PLoS neglected tropical diseases · 2014Article
- RC1339/APRc from Rickettsia conorii is a novel aspartic protease with properties of retropepsin-like enzymes.PLoS pathogens · 2014Article
- Multimethylation of Rickettsia OmpB catalyzed by lysine methyltransferases.The Journal of biological chemistry · 2014Article
- Inactivation of SAM-methyltransferase is the mechanism of attenuation of a historic louse borne typhus vaccine strain.PloS one · 2014Article
- Hemocytes from Pediculus humanus humanus are hosts for human bacterial pathogens.Frontiers in cellular and infection microbiology · 2014Article
- Article
- Two protein lysine methyltransferases methylate outer membrane protein B from Rickettsia.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rickettsia prowazekii, the agent of epidemic typhus, is an obligate intracellular bacterium that is transmitted to human beings by the body louse. Several strains that differ considerably in virulence are recognized, but the genetic basis for these variations has remained unknown since the initial description of the avirulent vaccine strain nearly 70 yr ago. We use a recently developed murine model of epidemic typhus and transcriptomic, proteomic, and genetic techniques to identify the factors associated with virulence. We identified four phenotypes of R. prowazekii that differed in virulence, associated with the up-regulation of antiapoptotic genes or the interferon I pathway in the host cells. Transcriptional and proteomic analyses of R. prowazekii surface protein expression and protein methylation varied with virulence. By sequencing a virulent strain and using comparative genomics, we found hotspots of mutations in homopolymeric tracts of poly(A) and poly(T) in eight genes in an avirulent strain that split and inactivated these genes. These included recO, putative methyltransferase, and exported protein. Passage of the avirulent Madrid E strain in cells or in experimental animals was associated with a cascade of gene reactivations, beginning with recO, that restored the virulent phenotype. An area of genomic plasticity appears to determine virulence in R. prowazekii and represents an example of adaptive mutation for this pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.