ArticleThe Journal of infectious diseases2010
The NH(2)-terminal region of Streptococcus pyogenes M5 protein confers protection against degradation by proteases and enhances mucosal colonization of mice.
Article in The Journal of infectious diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin.Acta crystallographica. Section D, Structural biology · 2020Article
- Structure-based design of broadly protective group a streptococcal M protein-based vaccines.Vaccine · 2017Article
- Conserved patterns hidden within group A Streptococcus M protein hypervariability recognize human C4b-binding protein.Nature microbiology · 2016Article
- Sequence variability is correlated with weak immunogenicity in Streptococcus pyogenes M protein.MicrobiologyOpen · 2015Article
- The nonideal coiled coil of M protein and its multifarious functions in pathogenesis.Advances in experimental medicine and biology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe NH(2)-terminal sequence of the M protein from group A streptococci defines the serotype of the organism and contains epitopes that evoke bactericidal antibodies.
methodsTo identify additional roles for this region of the M protein, we constructed a mutant of M5 group A streptococci expressing an M protein with a deletion of amino acid residues 3-22 (DeltaNH(2)).
resultsM5 streptococci and the DeltaNH(2) mutant were resistant to phagocytosis and were similarly virulent in mice. However, DeltaNH(2) was significantly less hydrophobic, contained less lipoteichoic acid on its surface, and demonstrated reduced adherence to epithelial cells. These differences were abolished when organisms were grown in the presence of protease inhibitors. Treatment with cysteine proteases or with human saliva resulted in the release of M protein from the DeltaNH(2) mutant at a significantly greater rate than observed with the wild-type M5 strain. Compared with the parent strain, the DeltaNH(2) strain also showed a significant reduction in its ability to colonize the upper respiratory mucosa of mice.
conclusionsThe NH(2) terminus of M5 protein has an important role in protecting the surface protein from proteolytic cleavage, thus preserving its function as an anchor for lipoteichoic acid, which is a primary mediator of adherence to epithelial cells and colonization.
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