ArticleJournal of the American Chemical Society2010
Decoding circulating nucleic acids in human serum using microfluidic single molecule spectroscopy.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Omega Nucleic Acids (ΩNA), Ultimate Nucleic Acids for Future Technology.Molecules (Basel, Switzerland) · 2026Review
- Circulating DNA fragmentomics and cancer screening.Cell genomics · 2023Review
- Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.Analytical chemistry · 2020Article
- Microfluidic Technologies for cfDNA Isolation and Analysis.Micromachines · 2019Review
- Recent advances in microfluidic methods in cancer liquid biopsy.Biomicrofluidics · 2019Review
- Versatile Analysis of DNA-Biomolecule Interactions in Solution by Hydrodynamic Separation and Single Molecule Detection.Analytical chemistry · 2019Article
- Fluorescence spectroscopic detection and measurement of single telomere molecules.Nucleic acids research · 2018Article
- Separation of sub-micron particles from micron particles using acoustic fluid relocation combined with acoustophoresis.Analytical and bioanalytical chemistry · 2018Article
- The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.Micromachines · 2018Review
- Advances in liquid biopsy on-chip for cancer management: Technologies, biomarkers, and clinical analysis.Critical reviews in clinical laboratory sciences · 2018Review
- On-chip wavelength multiplexed detection of cancer DNA biomarkers in blood.Biomicrofluidics · 2016Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
- Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution.Journal of the American Chemical Society · 2016Article
- Biomedical diagnosis perspective of epigenetic detections using alpha-hemolysin nanopore.AIMS materials science · 2015Article
- Direct interrogation of DNA content distribution in nanoparticles by a novel microfluidics-based single-particle analysis.Nano letters · 2014Article
- Point-of-care technologies for molecular diagnostics using a drop of blood.Trends in biotechnology · 2014Review
- Single-shot characterization of enzymatic reaction constants Km and kcat by an acoustic-driven, bubble-based fast micromixer.Analytical chemistry · 2012Article
- Micro total analysis systems for cell biology and biochemical assays.Analytical chemistry · 2012Review
- Unconventional Kondo effect in redox active single organic macrocyclic transistors.Journal of the American Chemical Society · 2011Article
- A single-layer, planar, optofluidic Mach-Zehnder interferometer for label-free detection.Lab on a chip · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Circulating nucleic acid (CNA) has been the focus of recent research as a noninvasive source of biomarker candidates. Among these markers, DNA fragment size has shown promise for discerning the source of CNA molecules in cancer and prenatal diagnostics. We have developed a one-step assay for analyzing circulating DNA size and quantity directly in human serum. Microfluidic cylindrical illumination confocal spectroscopy and fluorescence burst size analysis are used to individually count and size fluorescently-labeled CNA molecules as they are driven through a microfluidic constriction. First, single molecule sizing was performed on lambda Hind III digest DNA to obtain a size calibration curve. A linear relation between DNA length and burst size was seen from 564 bp to 27.5 kbp. Subsequently, the single molecule assay parameters were optimized. Finally, DNA sizing analysis was performed on serum samples from both early and late stage lung cancer patients. This assay was performed directly in patient serum using only a single reagent, a simple DNA intercalating dye, and without the need for DNA isolation or enzymatic amplification steps. This demonstrates that microfluidic single molecule spectroscopy can be a rapid, facile, and inexpensive alternative to the established PCR-based methods that have been used near exclusively for CNA analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.