ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2010
Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genetic basis of qualitative and quantitative resistance to powdery mildew in wheat: from consensus regions to candidate genes.BMC genomics · 2013Pooled it
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Frontiers in plant science · 2018Article
- Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Flow sorting and sequencing meadow fescue chromosome 4F.Plant physiology · 2013Article
- Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- FISHIS: fluorescence in situ hybridization in suspension and chromosome flow sorting made easy.PloS one · 2013Article
- Article
- Characterization of wheat DArT markers: genetic and functional features.Molecular genetics and genomics : MGG · 2012Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- DNA repair and crossing over favor similar chromosome regions as discovered in radiation hybrid of Triticum.BMC genomics · 2012Article
- Article
- Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe how the diversity arrays technology (DArT) can be coupled with chromosome sorting to increase the density of genetic maps in specific genome regions. Chromosome 3B and the short arm of chromosome 1B (1BS) of wheat were isolated by flow cytometric sorting and used to develop chromosome- and chromosome arm-enriched genotyping arrays containing 2,688 3B clones and 384 1BS clones. Linkage analysis showed that 553 of the 711 polymorphic 3B-derived markers (78%) mapped to chromosome 3B, and 59 of the 68 polymorphic 1BS-derived markers (87%) mapped to chromosome 1BS, confirming the efficiency of the chromosome-sorting approach. To demonstrate the potential for saturation of genetic maps, we constructed a consensus map of chromosome 3B using 19 mapping populations, including some that were genotyped with the 3B-enriched array. The 3B-derived DArT markers doubled the number of genetic loci covered. The resulting consensus map, probably the densest genetic map of 3B available to this date, contains 939 markers (779 DArTs and 160 other markers) that segregate on 304 genetically distinct loci. Importantly, only 2,688 3B-derived clones (probes) had to be screened to obtain almost twice as many polymorphic 3B markers (510) as identified by screening approximately 70,000 whole genome-derived clones (269). Since an enriched DArT array can be developed from less than 5 ng of chromosomal DNA, a quantity which can be obtained within 1 h of sorting, this approach can be readily applied to any crop for which chromosome sorting is available.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.