ArticleNature methods2010
Conditional gene expression and RNAi using MEC-8-dependent splicing in C. elegans.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The MEC-2E isoform with a large C-terminal completely rescues the touch sensation defect of C. elegans.Scientific reports · 2025Article
- Alternative splicing across the C. elegans nervous system.Nature communications · 2025Article
- MBL-1/Muscleblind regulates neuronal differentiation and controls the splicing of a terminal selector in Caenorhabditis elegans.PLoS genetics · 2024Article
- Muscleblind-1 interacts with tubulin mRNAs to regulate the microtubule cytoskeleton in C. elegans mechanosensory neurons.PLoS genetics · 2023Article
- Sensory neuron transcriptomes reveal complex neuron-specific function and regulation of mec-2/Stomatin splicing.Nucleic acids research · 2022Article
- TheGenetics · 2019Review
- Longevity: Lesson from Model Organisms.Genes · 2019Review
- The combinatorial control of alternative splicing in C. elegans.PLoS genetics · 2017Article
- Conserved binding of GCAC motifs by MEC-8, couch potato, and the RBPMS protein family.RNA (New York, N.Y.) · 2017Article
- Article
- Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover.Nucleic acids research · 2014Article
- Regulatory roles of RNA binding proteins in the nervous system of C. elegans.Frontiers in molecular neuroscience · 2014Review
- Article
- Article
- Role of the ubiquitin-proteasome system in nervous system function and disease: using C. elegans as a dissecting tool.Cellular and molecular life sciences : CMLS · 2012Review
- A growing molecular toolbox for the functional analysis of microRNAs in Caenorhabditis elegans.Briefings in functional genomics · 2011Review
- Caenorhabditis elegans aristaless/Arx gene alr-1 restricts variable gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Knock it down, switch it on.Nature methods · 2010Article
- From genes to function: the C. elegans genetic toolbox.Wiley interdisciplinary reviews. Developmental biologyReview
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Abstract
We describe a method for conditional regulation of gene expression based on the processing of an intron cassette. The RNA processing factor MEC-8 is necessary for the function of the Caenorhabditis elegans touch receptor neurons; mec-8 mutants are touch insensitive. We show here that this insensitivity involves the loss of MEC-8-dependent splicing of mec-2, which encodes a component of the mechanosensory transduction complex. MEC-8 is needed to remove the ninth intron in mec-2 pre-mRNA to form the longest of three mRNAs, mec-2a. Without MEC-8, splicing causes the termination of the transcript. Inclusion of mec-2 intron 9 is sufficient to convey mec-8-dependent regulation on other genes and, in mec-8(u218ts) mutants, resulted in their temperature-dependent expression. Because mec-8 is expressed ubiquitously in embryos and extensively in larvae, this system should produce temperature-sensitive expression for most genes. As an example, we report a strain that exhibits temperature-dependent RNA interference.
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