ArticleJournal of bacteriology2010
Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters.Nature structural & molecular biology · 2025Article
- Deorphanizing solute carriers inFrontiers in microbiology · 2024Article
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- A comparison of transporter gene expression in three species of Peronospora plant pathogens during host infection.PloS one · 2023Article
- Review
- On the Role of a Conserved Methionine in the NaNeurochemical research · 2022Article
- Unchanged nitrate and nitrite isotope fractionation during heterotrophic and Fe(II)-mixotrophic denitrification suggest a non-enzymatic link between denitrification and Fe(II) oxidation.Frontiers in microbiology · 2022Article
- Reductive evolution and unique predatory mode in the CPR bacterium Vampirococcus lugosii.Nature communications · 2021Article
- Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens.Current opinion in biotechnology · 2020Review
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Understanding transport processes in lichen,F1000Research · 2020Review
- Malate-Dependent Carbon Utilization Enhances Central Metabolism and Contributes to Biological Fitness ofFrontiers in microbiology · 2019Article
- Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility.Frontiers in microbiology · 2019Review
- Ammonia-oxidising archaea living at low pH: Insights from comparative genomics.Environmental microbiology · 2017Article
- C4-Dicarboxylate Utilization in Aerobic and Anaerobic Growth.EcoSal Plus · 2016Article
- The sensor kinase DctS forms a tripartite sensor unit with DctB and DctA for sensing C4-dicarboxylates in Bacillus subtilis.Journal of bacteriology · 2014Article
- Mechanism of transport modulation by an extracellular loop in an archaeal excitatory amino acid transporter (EAAT) homolog.The Journal of biological chemistry · 2013Article
- Article
- Ketoglutarate transport protein KgtP is secreted through the type III secretion system and contributes to virulence in Xanthomonas oryzae pv. oryzae.Applied and environmental microbiology · 2012Article
- Comparative genome analysis of central nitrogen metabolism and its control by GlnR in the class Bacilli.BMC genomics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial secondary transporters of the DctA family mediate ion-coupled uptake of C(4)-dicarboxylates. Here, we have expressed the DctA homologue from Bacillus subtilis in the Gram-positive bacterium Lactococcus lactis. Transport of dicarboxylates in vitro in isolated membrane vesicles was assayed. We determined the substrate specificity, the type of cotransported ions, the electrogenic nature of transport, and the pH and temperature dependence patterns. DctA was found to catalyze proton-coupled symport of the four C(4)-dicarboxylates from the Krebs cycle (succinate, fumurate, malate, and oxaloacetate) but not of other mono- and dicarboxylates. Because (i) succinate-proton symport was electrogenic (stimulated by an internal negative membrane potential) and (ii) the divalent anionic form of succinate was recognized by DctA, at least three protons must be cotransported with succinate. The results were interpreted in the light of the crystal structure of the homologous aspartate transporter Glt(Ph) from Pyrococcus horikoshii.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.