Evidence map›Paper›PMID 20363899›Full record

ArticleEukaryotic cell2010

Single-step affinity purification for fungal proteomics.

Hui-Lin Liu, Aysha H Osmani, Leena Ukil, Sunghun Son, Sarine Markossian, Kuo-Fang Shen, Meera Govindaraghavan, Archana Varadaraj, Shahr B Hashmi, Colin P De Souza and 1 more

Abstract read
In one paragraph

Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed.

  1. Article
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  13. SUMOlock reveals a more complete Aspergillus nidulans SUMOylome.Fungal genetics and biology : FG & B · 2019
    Article
  14. Article
  15. Article
  16. Article
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  18. mBio · 2017
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hui-Lin LiuDepartment of Molecular Genetics, Ohio State University, 496 W. 12th Ave., Columbus, OH 43210, USA.
Aysha H Osmani
Leena Ukil
Sunghun Son
Sarine Markossian
Kuo-Fang Shen
Meera Govindaraghavan
Archana Varadaraj
Shahr B Hashmi
Colin P De Souza
Stephen A Osmani

Funding

The role of the NIMA kinase in mitotic regulationR01GM042564 · NIGMS · WEIS CENTER FOR RESEARCH-GEISINGER CLINC · PI OSMANI, STEPHEN A · 1989 to 2013
$4.3M
NIGMS NIH HHS GM 42564NIGMS NIH HHS R01 GM042564
6 · The paper itself

Abstract

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

Indexed as

Aspergillus nidulansChromatography, AffinityElectrophoresis, Polyacrylamide GelEscherichia coliFungal ProteinsProteomicsSaccharomyces cerevisiaeFungal Proteins

Identifiers

PMID20363899
PMCPMC2863959

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.