ArticleAnalytical biochemistry2010
Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Andes virus and the aging host: lessons from the 2026 outbreak and a framework for age-related disease severity.GeroScience · 2026Review
- Pathogenicity and virulence of Rodent-Borne Orthohantaviruses.Virulence · 2025Review
- Ultracentrifugation Purification of Cache Valley Virus Using Iodixanol.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Replication kinetics of pathogenic Eurasian orthohantaviruses in human mesangial cells.Virology journal · 2024Article
- Vascular dysfunction in hemorrhagic viral fevers: opportunities for organotypic modeling.Biofabrication · 2024Review
- Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses.Viruses · 2023Article
- Expression Profile of Human Renal Mesangial Cells Is Altered by Infection with Pathogenic Puumala Orthohantavirus.Viruses · 2022Article
- Integrin activation is an essential component of SARS-CoV-2 infection.Scientific reports · 2021Article
- Cells of the human respiratory tract support the replication of pathogenic Old World orthohantavirus Puumala.Virology journal · 2021Article
- Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells.eLife · 2021Article
- Hantavirus entry: Perspectives and recent advances.Advances in virus research · 2019Article
- Article
- A High-Throughput Flow Cytometry Screen Identifies Molecules That Inhibit Hantavirus Cell Entry.SLAS discovery : advancing life sciences R & D · 2018Article
- Low-affinity binding inMolecular biology of the cell · 2017Article
- The Experimental Research (In Vitro) of Carrageenans and Fucoidans to Decrease Activity of Hantavirus.Food and environmental virology · 2016Article
- A Molecular-Level Account of the Antigenic Hantaviral Surface.Cell reports · 2016Article
- Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.PloS one · 2016Article
- Anti-infective immunoadhesins from plants.Plant biotechnology journal · 2015Review
- Elevated cytokines, thrombin and PAI-1 in severe HCPS patients due to Sin Nombre virus.Viruses · 2015Article
- Equilibrium and kinetics of Sin Nombre hantavirus binding at DAF/CD55 functionalized bead surfaces.Viruses · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Hantaviruses cause two severe diseases in humans: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The lack of vaccines or specific drugs to prevent or treat HFRS and HCPS and the requirement for conducting experiments in a biosafety level 3 laboratory (BSL-3) limit the ability to probe the mechanism of infection and disease pathogenesis. In this study, we developed a generalizable spectroscopic assay to quantify saturable fluorophore sites solubilized in envelope membranes of Sin Nombre virus (SNV) particles. We then used flow cytometry and live cell confocal fluorescence microscopy imaging to show that ultraviolet (UV)-killed SNV particles bind to the cognate receptors of live virions, namely, decay accelerating factor (DAF/CD55) expressed on Tanoue B cells and alpha(v)beta(3) integrins expressed on Vero E6 cells. SNV binding to DAF is multivalent and of high affinity (K(d) approximately 26pM). Self-exchange competition binding assays between fluorescently labeled SNV and unlabeled SNV are used to evaluate an infectious unit-to-particle ratio of approximately 1:14,000. We configured the assay for measuring the binding of fluorescently labeled SNV to Tanoue B suspension cells using a high-throughput flow cytometer. In this way, we established a proof-of-principle high-throughput screening (HTS) assay for binding inhibition. This is a first step toward developing HTS format assays for small molecule inhibitors of viral-cell interactions as well as dissecting the mechanism of infection in a BSL-2 environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.