ArticleCryobiology2010
Comparison between the temperatures of intracellular ice formation in fresh mouse oocytes and embryos and those previously subjected to a vitrification procedure.
Article in Cryobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 6 citations in OpenAlex.
- Effect of the expression of aquaporins 1 and 3 in mouse oocytes and compacted eight-cell embryos on the nucleation temperature for intracellular ice formation.Reproduction (Cambridge, England) · 2011Article
- The temperature and type of intracellular ice formation in preimplantation mouse embryos as a function of the developmental stage.Biology of reproduction · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When cells that have been subjected to supposedly innocuous freezing or vitrification procedures are used as the source material for subsequent experiments, it is important that they possess or exhibit the same relevant properties as fresh cells. In this study, we compared the temperatures of intracellular ice formation (IIF) in previously vitrified mouse oocytes/embryos with those in fresh intact ones. In the case of MII oocytes, 2-cell embryos, 4-6-cell embryos, and morulae, there are no significant differences (p>0.05); namely, -33.3 degrees C (fresh) vs. -35.4 degrees C (vitrified) with MII oocytes, -40.6 degrees C (fresh) vs. -38.7 degrees C (vitrified) with 2-cell embryos, -38.0 degrees C (fresh) vs. -39.4 degrees C (vitrified) with 4-6-cell embryos, -24.5 degrees C (fresh) vs. -24.2 degrees C (vitrified) with morulae. But, in 8-cell embryos, there is a significant difference (p<0.05) between fresh (-37.9 degrees C) and vitrified (-32.9 degrees C). If we include this significant difference, the overall IIF temperature of fresh cells is 0.74 degrees C lower than that of previously vitrified cells. If we exclude it, the IIF temperature for fresh cells is 0.32 degrees C higher than that for previously vitrified cells. Our conclusion then is that there is no difference between the IIF temperatures of fresh and previously vitrified cells.
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Registered trials
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