ArticleJournal of the American Chemical Society2010
A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.
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Who cites it
157 citing papers in PubMed, 511 citations in OpenAlex.
- OCancer cell · 2017Trial
- Beyond DCFH-DA: A Critical Review of Hydrogen Peroxide and Superoxide Detection Strategies in Mammalian Living Systems (2015-2026).International journal of molecular sciences · 2026Review
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- Essential Oils as Antioxidants: Mechanistic Insights from Radical Scavenging to Redox Signaling.Antioxidants (Basel, Switzerland) · 2025Review
- In vivo imaging of reactive oxygen species after myocardial ischemia-reperfusion injury: a large animal multimodal imaging and transcriptomic study.bioRxiv : the preprint server for biology · 2025Article
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- A single-molecule platform for photo-triggered peroxynitrite release with built-in fluorescence calibration.Smart molecules : open access · 2025Article
- SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses.The Plant journal : for cell and molecular biology · 2025Article
- Phenyl Radical-Mediated Fluorogenic Cyclization for Specific Detection of Peroxynitrite.Analytical chemistry · 2025Article
- Fluorescein-based SynNotch adaptors for regulating gene expression responses to diverse extracellular and matrix-based cues.Nature communications · 2025Article
- Non-thermal plasma activated water is an effective nitrogen fertilizer alternative for Arabidopsis thaliana.PloS one · 2025Article
- Fluorogenic chemical tools to shed light on CES1-mediated adverse drug interactions.Chemical communications (Cambridge, England) · 2024Article
- Hydrogen peroxide responsive theranostics for cancer-selective activation of DNA alkylators and real-time fluorescence monitoring in living cells.European journal of medicinal chemistry · 2024Article
- Mitogen-Activated Protein Kinase Kinase OsMEK2 Positively Regulates CaAntioxidants (Basel, Switzerland) · 2024Article
- Modulating and Imaging Macrophage Reprogramming for Cancer Immunotherapy.Phenomics (Cham, Switzerland) · 2024Review
- An Expanded Palette of Fluorescent COS/HAngewandte Chemie (International ed. in English) · 2024Article
- A novel ruthenium-silver based antimicrobial potentiates aminoglycoside activity againstmSphere · 2023Article
97 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We present a new family of fluorescent probes with varying emission colors for selectively imaging hydrogen peroxide (H(2)O(2)) generated at physiological cell signaling levels. This structurally homologous series of fluorescein- and rhodol-based reporters relies on a chemospecific boronate-to-phenol switch to respond to H(2)O(2) over a panel of biologically relevant reactive oxygen species (ROS) with tunable excitation and emission maxima and sensitivity to endogenously produced H(2)O(2) signals, as shown by studies in RAW264.7 macrophages during the phagocytic respiratory burst and A431 cells in response to EGF stimulation. We further demonstrate the utility of these reagents in multicolor imaging experiments by using one of the new H(2)O(2)-specific probes, Peroxy Orange 1 (PO1), in conjunction with the green-fluorescent highly reactive oxygen species (hROS) probe, APF. This dual-probe approach allows for selective discrimination between changes in H(2)O(2) and hypochlorous acid (HOCl) levels in live RAW264.7 macrophages. Moreover, when macrophages labeled with both PO1 and APF were stimulated to induce an immune response, we discovered three distinct types of phagosomes: those that generated mainly hROS, those that produced mainly H(2)O(2), and those that possessed both types of ROS. The ability to monitor multiple ROS fluxes simultaneously using a palette of different colored fluorescent probes opens new opportunities to disentangle the complex contributions of oxidation biology to living systems by molecular imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.