ArticlePloS one2010
An expanded self-antigen peptidome is carried by the human lymph as compared to the plasma.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 62 citations in OpenAlex.
- Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics.Journal of proteomics · 2013Trial
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- Infection control in the brain and the eye.Acta ophthalmologica · 2026Review
- Development and validation of a gastric cancer prognostic model utilizing lymphatic endothelial cell-related genes.Diagnostic pathology · 2025Article
- Multi-site Ultrasound-guided Fine Needle Aspiration to Study Cells and Soluble Factors From Human Lymph Nodes.Current protocols · 2024Article
- Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load.Cell reports · 2024Article
- Same yet different - how lymph node heterogeneity affects immune responses.Nature reviews. Immunology · 2024Review
- Nanotechnologies for Physiology-Informed Drug Delivery to the Lymphatic System.Annual review of biomedical engineering · 2023Review
- Hepatic lymphatic vascular system in health and disease.Journal of hepatology · 2022Review
- Information flow in the spatiotemporal organization of immune responses.Immunological reviews · 2022Review
- Molecular Determinants Regulating the Plasticity of the MHC Class II Immunopeptidome.Frontiers in immunology · 2022Review
- Review
- Specialized Pro-Resolving Mediators and the Lymphatic System.International journal of molecular sciences · 2021Review
- Coagulation in Lymphatic System.Frontiers in cardiovascular medicine · 2021Review
- Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients.The Journal of experimental medicine · 2019Article
- Contribution of the plasma and lymph Degradome and Peptidome to the MHC Ligandome.Immunogenetics · 2019Review
- In vitro model reveals a role for mechanical stretch in the remodeling response of lymphatic muscle cells.Microcirculation (New York, N.Y. : 1994) · 2019Article
- The Antigen Processing and Presentation Machinery in Lymphatic Endothelial Cells.Frontiers in immunology · 2019Review
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe pre-nodal afferent lymph is the fluid which directly derives from the extracellular milieu from every parenchymal organ and, as it continues to circulate between the cells, it collects products deriving from the organ metabolism/catabolism. A comprehensive qualitative and quantitative investigation of the self-antigenic repertoire transported by the human lymph is still missing. METHODOLOGY/PRINCIPAL
findingsA major difference between lymph and plasma could be visualized by FPLC and 2D gel in the amount of low molecular weight products corresponding to peptide fragments. Naturally processed peptides in normal pre-nodal human lymph were then fractionated by HPLC and characterized by multidimensional mass spectrometry. Analysis of more then 300 sequences identified self-peptides derived from both intracellular and extracellular proteins revealing the variety of catabolic products transported by human lymph. Quantitative analysis established that at least some of these peptides are present in the circulating lymph in nanomolar concentration. CONCLUSIONS/SIGNIFICANCE: The peptidome, generated by physiological tissue catabolism and transported by the pre-nodal lymph, is in addition to the self-peptidome generated in endosomal compartment. Unlike self antigen processed by local or nodal APC, which mostly produce epitopes constrained by the endosomal processing activity, self antigens present in the lymph could derived from a wider variety of processing pathways; including caspases, involved in cellular apoptosis, and ADAM and other metalloproteinases involved in surface receptor editing, cytokines processing and matrix remodeling. Altogether, expanding the tissue-specific self-repertoire available for the maintenance of immunological tolerance.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.