ArticleNature protocols2010
Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 162 citations in OpenAlex.
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- On-Tip Polymerization Method for Multimodal Characterization of Nanoparticles with Electron/Ion Imaging and Atom Probe Tomography.Small methods · 2026Article
- Reactive Oxygen Species Scavenging Capacity of Functional Fullerenes in Solution and in Macrophage Cells.ACS applied nano materials · 2024Article
- Transport of Nanoparticles into Plants and Their Detection Methods.Nanomaterials (Basel, Switzerland) · 2024Review
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- Zinc-Based Nanoparticles, but Not Silicon-Based Nanoparticles, Accumulate in Mitochondria and Promote Cell Death in Liver Cancer Cells.International journal of nanomedicine · 2024Article
- Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.eNeuro · 2023Article
- Fe-Cr-Nb-B Magnetic Particles and Adipose-Derived Mesenchymal Cells Trigger Cancer Cell Apoptosis by Magneto-Mechanical Actuation.Nanomaterials (Basel, Switzerland) · 2023Article
- Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.bioRxiv : the preprint server for biology · 2023Article
- Cellular uptake and retention studies of silica nanoparticles utilizing senescent fibroblasts.Scientific reports · 2023Article
- Fe-Cr-Nb-B Ferrofluid for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2022Article
- Pomegranate peel induced biogenic synthesis of silver nanoparticles and their multifaceted potential against intracellular pathogen and cancer.Saudi journal of biological sciences · 2021Article
- Biosynthesis of silver nanoparticles using Haloferax sp. NRS1: image analysis, characterization, in vitro thrombolysis and cytotoxicity.AMB Express · 2021Article
- Surface-bound reactive oxygen species generating nanozymes for selective antibacterial action.Nature communications · 2021Article
- Pathogenesis ofVirulence · 2020Article
- Engineering magnetic nanoparticles and their integration with microfluidics for cell isolation.Journal of colloid and interface science · 2020Article
- Discrimination of radiosensitive and radioresistant murine lymphoma cells by Raman spectroscopy and SERS.Biomedical optics express · 2020Article
- Curcumin and Solid Lipid Curcumin Particles Induce Autophagy, but Inhibit Mitophagy and the PI3K-Akt/mTOR Pathway in Cultured Glioblastoma Cells.International journal of molecular sciences · 2019Article
- Functionalized Keratin as Nanotechnology-Based Drug Delivery System for the Pharmacological Treatment of Osteosarcoma.International journal of molecular sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the use of transmission electron microscopy (TEM) for cellular ultrastructural examination of nanoparticle (NP)-exposed biomaterials. Preparation and imaging of electron-transparent thin cell sections with TEM provides excellent spatial resolution (approximately 1 nm), which is required to track these elusive materials. This protocol provides a step-by-step method for the mass-basis dosing of cultured cells with NPs, and the process of fixing, dehydrating, staining, resin embedding, ultramicrotome sectioning and subsequently visualizing NP uptake and translocation to specific intracellular locations with TEM. In order to avoid potential artifacts, some technical challenges are addressed. Based on our results, this procedure can be used to elucidate the intracellular fate of NPs, facilitating the development of biosensors and therapeutics, and provide a critical component for understanding NP toxicity. This protocol takes approximately 1 week.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.