ArticleGenome research2010
mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus.
Article in Genome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 155 citations in OpenAlex.
- Article
- A giant virus forms a specialized subcellular environment within its amoeba host for efficient translation.Nature microbiology · 2026Article
- Giant DNA viruses encode a hallmark translation initiation complex of eukaryotic life.bioRxiv : the preprint server for biology · 2025Article
- Mimivirus transcription and translation occur at well-defined locations within amoeba host cells.Journal of virology · 2025Article
- Virocell resource manipulation under nutrient limitation.mSystems · 2025Article
- Ultrastructural and transcriptional changes during a giant virus infection of a green alga.Npj viruses · 2025Article
- Article
- Sputnik virophage disrupts the transcriptional regulation of its host giant virus.Journal of virology · 2025Article
- Cytochrome b5 occurrence in giant and other viruses belonging to the phylum Nucleocytoviricota.Npj viruses · 2025Article
- Acanthamoeba polyphaga de novo transcriptome and its dynamics during Mimivirus infection.Scientific reports · 2024Article
- Complex transcriptional regulations of a hyperparasitic quadripartite system in giant viruses infecting protists.Nature communications · 2024Article
- Metatranscriptomic analysis reveals dissimilarity in viral community activity between an ice-free and ice-covered winter in Lake Erie.mSystems · 2024Article
- Giant viral signatures on the Greenland ice sheet.Microbiome · 2024Article
- Endogenous virophages are active and mitigate giant virus infection in the marine protistProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Virologs, viral mimicry, and virocell metabolism: the expanding scale of cellular functions encoded in the complex genomes of giant viruses.FEMS microbiology reviews · 2023Article
- Review
- Megaviruses contain various genes encoding for eukaryotic vesicle trafficking factors.Traffic (Copenhagen, Denmark) · 2022Article
- Role of an FNIP Repeat Domain-Containing Protein Encoded by Megavirus Baoshan during Viral Infection.Journal of virology · 2022Article
- Giant viruses of themicroLife · 2022Article
- Diversity and genomics of giant viruses in the North Pacific Subtropical Gyre.Frontiers in microbiology · 2022Article
36 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mimivirus, a virus infecting Acanthamoeba, is the prototype of the Mimiviridae, the latest addition to the nucleocytoplasmic large DNA viruses. The Mimivirus genome encodes close to 1000 proteins, many of them never before encountered in a virus, such as four amino-acyl tRNA synthetases. To explore the physiology of this exceptional virus and identify the genes involved in the building of its characteristic intracytoplasmic "virion factory," we coupled electron microscopy observations with the massively parallel pyrosequencing of the polyadenylated RNA fractions of Acanthamoeba castellanii cells at various time post-infection. We generated 633,346 reads, of which 322,904 correspond to Mimivirus transcripts. This first application of deep mRNA sequencing (454 Life Sciences [Roche] FLX) to a large DNA virus allowed the precise delineation of the 5' and 3' extremities of Mimivirus mRNAs and revealed 75 new transcripts including several noncoding RNAs. Mimivirus genes are expressed across a wide dynamic range, in a finely regulated manner broadly described by three main temporal classes: early, intermediate, and late. This RNA-seq study confirmed the AAAATTGA sequence as an early promoter element, as well as the presence of palindromes at most of the polyadenylation sites. It also revealed a new promoter element correlating with late gene expression, which is also prominent in Sputnik, the recently described Mimivirus "virophage." These results-validated genome-wide by the hybridization of total RNA extracted from infected Acanthamoeba cells on a tiling array (Agilent)--will constitute the foundation on which to build subsequent functional studies of the Mimivirus/Acanthamoeba system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.