ArticleCold Spring Harbor protocols2010
Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection.
Article in Cold Spring Harbor protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Article
- FORTIS: a live-cell assay to monitor AMPA receptors using pH-sensitive fluorescence tags.Translational psychiatry · 2021Article
- The Property-Based Practical Applications and Solutions of Genetically Encoded Acetylcholine and Monoamine Sensors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Review
- Genetically encoded sensors enable micro- and nano-scopic decoding of transmission in healthy and diseased brains.Molecular psychiatry · 2021Review
- MERLIN: a novel BRET-based proximity biosensor for studying mitochondria-ER contact sites.Life science alliance · 2020Article
- Early Effects of Aβ Oligomers on Dendritic Spine Dynamics and Arborization in Hippocampal Neurons.Frontiers in synaptic neuroscience · 2020Article
- Neurogranin Regulates Metaplasticity.Frontiers in molecular neuroscience · 2019Article
- Ras and Rap Signal Bidirectional Synaptic Plasticity via Distinct Subcellular Microdomains.Neuron · 2018Article
- Synaptic Vesicle Endocytosis in Different Model Systems.Frontiers in cellular neuroscience · 2018Review
- MAP1B Light Chain Modulates Synaptic Transmission via AMPA Receptor Intracellular Trapping.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Multitype Bellman-Harris branching model provides biological predictors of early stages of adult hippocampal neurogenesis.BMC systems biology · 2017Article
- Presynaptic serotonin 2A receptors modulate thalamocortical plasticity and associative learning.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Hippocampal Dendritic Spines Are Segregated Depending on Their Actin Polymerization.Neural plasticity · 2016Article
- A New Defective Helper RNA to Produce Recombinant Sindbis Virus that Infects Neurons but does not Propagate.Frontiers in neuroanatomy · 2016Article
- LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery.The Journal of cell biology · 2015Article
- Neuroanatomy goes viral!Frontiers in neuroanatomy · 2015Review
- Quantitative phosphoproteomics unravels biased phosphorylation of serotonin 2A receptor at Ser280 by hallucinogenic versus nonhallucinogenic agonists.Molecular & cellular proteomics : MCP · 2014Article
- Fluorescent labeling of dendritic spines in cell cultures with the carbocyanine dye "DiI".Frontiers in neuroanatomy · 2014Article
- MAP1B-dependent Rac activation is required for AMPA receptor endocytosis during long-term depression.The EMBO journal · 2013Article
- Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI).Neuron · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Expression of green fluorescent protein (GFP), its more fluorescent mutant forms (e.g., EGFP [enhanced GFP]), or their fusion protein derivatives, affords a number of informative possibilities in cellular neuroscience. EGFP is a soluble protein and appears to be homogeneously distributed within the cytosol of neurons when expressed. Thus, it reveals the structure of the neuron, including the cell body, and axonal and dendritic arbors. It is also sufficiently bright to reveal detailed structures such as axonal boutons and dendritic spines. When expressed as a fusion protein, EGFP can provide information about the distribution characteristics of the proteins within neurons. Furthermore, during single-cell electrophysiological studies, such expression can direct the investigator to record from a cell carrying a foreign gene. In this protocol, we describe the use of the Sindbis pseudovirus expression system to deliver GFP to neurons. Sindbis is a member of the alphaviruses, which are plus-stranded RNA viruses. This protocol uses the DH(26S) strain, which preferentially infects neurons over glia (50:1). Two infection methods are given: one for dissociated hippocampal cultured neurons and one for organotypic hippocampal slices.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.