ReviewJournal of fluorescence2010
The concept of λ-ratiometry in fluorescence sensing and imaging.
Review in Journal of fluorescence, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.Analytical chemistry · 2025Article
- Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera.Light, science & applications · 2025Article
- High-throughput investigation of macromolecular interactions for drug development using spectral shift technology.Biophysical reviews · 2025Review
- Modular access to nucleobase GFP-surrogates: pH-responsive smart probes for ratiometric nucleic acid diagnostics.Chemical science · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Ratiometric Fluorescent Sensors Illuminate Cellular Magnesium Imbalance in a Model of Acetaminophen-Induced Liver Injury.Journal of the American Chemical Society · 2023Article
- Simulating the Voltage-Dependent Fluorescence of Di-8-ANEPPS in a Lipid Membrane.The journal of physical chemistry letters · 2023Article
- Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.Communications chemistry · 2023Article
- Multi-parametric sensing by multi-channel molecular fluorescent probes based on excited state intramolecular proton transfer and charge transfer processes.BBA advances · 2023Article
- Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe.Molecules (Basel, Switzerland) · 2022Article
- Fluorescent RET-Based Chemosensor Bearing 1,8-Naphthalimide and Styrylpyridine Chromophores for Ratiometric Detection of HgBiosensors · 2022Article
- Effects of Cations on HPTS Fluorescence and Quantification of Free Gadolinium Ions in Solution; Assessment of Intracellular Release of GdMolecules (Basel, Switzerland) · 2022Article
- A facile combinatorial approach to construct a ratiometric fluorescent sensor: application for the real-time sensing of cellular pH changes.Chemical science · 2021Article
- Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor.Sensors (Basel, Switzerland) · 2021Article
- Probing of Nucleic Acid Structures, Dynamics, and Interactions With Environment-Sensitive Fluorescent Labels.Frontiers in chemistry · 2020Review
- A Near-Infrared Ratiometric Fluorescent Probe for Highly Selective Recognition and Bioimaging of Cysteine.Frontiers in chemistry · 2019Article
- Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore.Chemical science · 2018Article
- Ratiometric optical nanoprobes enable accurate molecular detection and imaging.Chemical Society reviews · 2018Review
- Chromis-1, a Ratiometric Fluorescent Probe Optimized for Two-Photon Microscopy Reveals Dynamic Changes in Labile Zn(II) in Differentiating Oligodendrocytes.ACS sensors · 2018Article
- Coumarin-Based Fluorescent Probe for Hypochlorites and Real Application in Tap Water.Journal of fluorescence · 2017Article
Corrections and comments
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Authors and funding
1 author.
Funding
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Abstract
Very limited number of parameters is available for fluorescence sensing and imaging. The changes of intensity are of low analytical value due to the absence of internal reference. Anisotropy and lifetime sensing have their own limitations. In this respect the λ-ratiometric (based on intensity ratios at two or more wavelengths) recording of spectral changes becomes more popular. Because the spectral changes are connected directly with the variations of interaction energies this approach is seen as the most universal method to study intermolecular interactions. It is applicable for different sensor formats and for obtaining analytical information from cell images. Here we critically analyze different approaches in λ-ratiometric sensing that use single and double fluorescence emitters and are based on different mechanisms producing spectroscopic change. Very promising is the exploration of mechanisms that allow obtaining ratiometric response from a single dye.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.