ArticleJournal of assisted reproduction and genetics2010
Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints.
Article in Journal of assisted reproduction and genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Genome instability in mammalian embryos implications for genome editing, development, and evolution.Current opinion in genetics & development · 2025Review
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- Predicting patient outcomes with gene-expression biomarkers from colorectal cancer organoids and cell lines.Frontiers in molecular biosciences · 2025Article
- Microarray evidence that 8-cell human embryos express some hormone family members including oxytocin.Journal of assisted reproduction and genetics · 2024Article
- Spectral and Redox Properties of a Recombinant Mouse CytochromeMolecules (Basel, Switzerland) · 2023Article
- Genetic mosaicism in the human brain: from lineage tracing to neuropsychiatric disorders.Nature reviews. Neuroscience · 2022Review
- Molecular contribution to embryonic aneuploidy and karyotypic complexity in initial cleavage divisions of mammalian development.Development (Cambridge, England) · 2022Article
- The uncertain science of preimplantation and prenatal genetic testing.Nature medicine · 2022Article
- Review
- The landscape of somatic mutation in cerebral cortex of autistic and neurotypical individuals revealed by ultra-deep whole-genome sequencing.Nature neuroscience · 2021Article
- The impacts of the number of prefreeze and postthaw blastomeres on embryo implantation potential: A systematic analysis.Medicine · 2020Article
- Human germline genome editing.Nature cell biology · 2019Review
- Review
- Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts.Scientific reports · 2018Article
- Early human embryos are naturally aneuploid-can that be corrected?Journal of assisted reproduction and genetics · 2017Review
- Unraveling the association between genetic integrity and metabolic activity in pre-implantation stage embryos.Scientific reports · 2016Article
- On becoming accepting of the imperfectionsin mammalian embryogenesis.Journal of assisted reproduction and genetics · 2016Article
- Microarray Analyses Reveal Marked Differences in Growth Factor and Receptor Expression Between 8-Cell Human Embryos and Pluripotent Stem Cells.Stem cells and development · 2016Article
- Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences.Cell and tissue research · 2016Review
- Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis.Nature communications · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo understand cell cycle controls in the 8-Cell human blastomere.
methodsData from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripotency, and embryonic stem cells. A sub database of 3,803 genes identified by high throughput RNA knock-down studies, plus genes that oscillate in human cells, was analyzed.
resultsThirty-five genes over-detected at least 7-fold specifically on the 8-Cell arrays were enriched for cell cycle drivers and for proteins that stabilize chromosome cohesion and spindle attachment and limit DNA and centrosome replication to once per cycle.
conclusionsThese results indicate that 8-cell human blastomere cleavage is guided by cyclic over-expression of key proteins, rather than canonical checkpoints, leading to rapidly increasing gene copy number and a susceptibility to chromosome and cytokinesis mishaps, well-noted characteristics of preimplantation human embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.