Novel method of single antibiotic selection of cells containing multiple stage-specific promoter-fluorophore plasmids.

Achuta K Guddati, John A Kessler

Abstract read

In one paragraph

Article in Journal of biomolecular techniques : JBT, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Achuta K GuddatiDepartment of Internal Medicine, Harlem Hospital Center at Columbia University, New York, New York 10037, USA. ag2876@columbia.edu

John A Kessler

Funding

REGULATION OF NEUROTRANSMITTER METABOLISMR01NS020778 · NINDS · YESHIVA UNIVERSITY · PI KESSLER, JOHN A · 1985 to 2014

$4.6M

MECHANISMS GOVERNING NEURONAL DEVELOPMENTR01NS020013 · NINDS · YESHIVA UNIVERSITY · PI KESSLER, JOHN A · 1985 to 2013

$4.1M

NINDS NIH HHS R01 NS020013NINDS NIH HHS R01 NS020778

6 · The paper itself

Abstract

This study demonstrates a novel method by which multiple separate plasmids can be stably integrated into the genome using single antibiotic resistance for selection. This method was used to integrate three different cardiac-specific promoters driving different fluorophores into murine embryonic stem cells, allowing sequential visualization of various stages of cardiac differentiation. This method is broadly applicable to the study of cell lineage of different stem/progenitor cells.

Indexed as

Genetic TechniquesAnimalsCell SeparationFluorescent DyesHeartHumansIndolesMesenchymal Stem CellsMiceMicroscopy, Phase-ContrastMyocytes, CardiacNeomycinOrgan SpecificityPlasmidsPromoter Regions, GeneticProtein MultimerizationDAPIecdysone receptorFluorescent DyesIndolesNeomycinReceptors, Retinoic AcidReceptors, Steroiddifferentiation

Identifiers

PMID20357978

PMCPMC2841996

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.