ArticleBiology of reproduction2010
Human endometrial stromal cell rho GTPases have opposing roles in regulating focal adhesion turnover and embryo invasion in vitro.
Article in Biology of reproduction, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos.PloS one · 2012Trial
- Genome-Wide Association Study of Daughter Pregnancy Rate in Crossbred Dairy Cows.International journal of molecular sciences · 2025Article
- Unlocking TB-COPD Pathogenesis: A Biotechnological Approach Targeting the PI3K/Akt Pathway.Iranian journal of biotechnology · 2025Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Molecular and Physiological Aspects of SARS-CoV-2 Infection in Women and Pregnancy.Frontiers in global women's health · 2022Review
- Interaction between insulin and androgen signalling in decidualization, cell migration and trophoblast invasion in vitro.Journal of cellular and molecular medicine · 2021Article
- Organoids to model the endometrium: implantation and beyond.Reproduction & fertility · 2021Review
- Genetic and epigenetic changes in the eutopic endometrium of women with endometriosis: association with decreased endometrial αvβ3 integrin expression.Molecular human reproduction · 2021Article
- Transcriptome, Spliceosome and Editome Expression Patterns of the Porcine Endometrium in Response to a Single Subclinical Dose ofInternational journal of molecular sciences · 2020Article
- Estrogen-induced FOS-like 1 regulates matrix metalloproteinase expression and the motility of human endometrial and decidual stromal cells.The Journal of biological chemistry · 2020Article
- Loss of CDYL Results in Suppression of CTNNB1 and Decreased Endometrial Receptivity.Frontiers in cell and developmental biology · 2020Article
- miR-29c overexpression and COL4A1 downregulation in infertile human endometrium reduces endometrial epithelial cell adhesive capacity in vitro implying roles in receptivity.Scientific reports · 2019Article
- The Notch Family Transcription Factor, RBPJκ, Modulates Glucose Transporter and Ovarian Steroid Hormone Receptor Expression During Decidualization.Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Inside the Endometrial Cell Signaling Subway: Mind the Gap(s).International journal of molecular sciences · 2018Review
- RBPJ mediates uterine repair in the mouse and is reduced in women with recurrent pregnancy loss.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Prokineticin 1 is up-regulated by insulin in decidualizing human endometrial stromal cells.Journal of cellular and molecular medicine · 2018Article
- Integrin beta8 (ITGB8) activates VAV-RAC1 signaling via FAK in the acquisition of endometrial epithelial cell receptivity for blastocyst implantation.Scientific reports · 2017Article
- Extended Culture of Encapsulated Human Blastocysts in Alginate Hydrogel Containing Decidualized Endometrial Stromal Cells in the Presence of Melatonin.Molecular biotechnology · 2016Article
- Ancient transposable elements transformed the uterine regulatory landscape and transcriptome during the evolution of mammalian pregnancy.Cell reports · 2015Article
- Implantation and Establishment of Pregnancy in Human and Nonhuman Primates.Advances in anatomy, embryology, and cell biology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Implantation of the embryo into the uterine compartment is a multistep event involving attachment of the embryo to the endometrial epithelia, followed by invasion of the embryo through the endometrial stroma. RHOA, RAC1, and CDC42 are members of the Rho GTPase family of proteins, which control cell functions such as cell migration and cytoskeletal reorganization. Herein, using a heterologous in vitro coculture model, we show that implantation of mouse blastocysts into human endometrial stromal cells (hESCs) is regulated by Rho GTPase activity in hESCs. Whereas iRNA-mediated silencing of RAC1 expression in hESCs led to inhibition of embryo implantation, silencing of either RHOA or CDC42 in hESCs promoted embryo implantation in coculture assays. Analysis of downstream signaling pathways demonstrated that RAC1 silencing was associated with decreased focal adhesion disassembly and resulted in large focal adhesion complexes in hESCs. In contrast, RHOA or CDC42 silencing resulted in perturbed focal adhesion assembly, leading to a decrease in the number of focal adhesions observed. Furthermore, inhibition of Rho signaling using a Rho kinase inhibitor, Y27632, led to decreased activation of protein tyrosine kinase 2 (PTK2, also called focal adhesion kinase) and decreased focal adhesion assembly. Importantly, perturbation of focal adhesion turnover in hESCs, mediated by PTK2 silencing, resulted in inhibition of embryo implantation into hESC monolayers. These findings suggest that Rho GTPase-PTK2-dependent remodeling of the endometrial stromal cell compartment may be critical for successful embryo implantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.