ArticlePlant physiology2010
Agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- A polygalacturonase gene PG031 regulates seed coat permeability with a pleiotropic effect on seed weight in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- A tau class GST, OsGSTU5, interacts with VirE2 and modulates the Agrobacterium-mediated transformation in rice.Plant cell reports · 2022Article
- Microautophagy Mediates Vacuolar Delivery of Storage Proteins in Maize Aleurone Cells.Frontiers in plant science · 2022Article
- Functional Characterization of Cotton C-Repeat Binding Factor Genes Reveal Their Potential Role in Cold Stress Tolerance.Frontiers in plant science · 2021Article
- Transient expression of fluorescently tagged proteins in developing maize aleurone cells.MethodsX · 2021Article
- Optimization of isolation and transfection conditions of maize endosperm protoplasts.Plant methods · 2020Article
- Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Biotechnology for biofuels · 2019Article
- Subcellular localization of Arabidopsis arogenate dehydratases suggests novel and non-enzymatic roles.Journal of experimental botany · 2017Article
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
- Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156.Scientific reports · 2016Article
- Identification of Transcription Factors ZmMYB111 and ZmMYB148 Involved in Phenylpropanoid Metabolism.Frontiers in plant science · 2016Article
- Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Current perspectives on the hormonal control of seed development in Arabidopsis and maize: a focus on auxin.Frontiers in plant science · 2014Review
- Article
- A novel method of transgene delivery into triticale plants using the Agrobacterium transferred DNA-derived nano-complex.Plant physiology · 2012Article
- The thick aleurone1 mutant defines a negative regulation of maize aleurone cell fate that functions downstream of defective kernel1.Plant physiology · 2011Article
- Delivery of prolamins to the protein storage vacuole in maize aleurone cells.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing maize (Zea mays) endosperms can be excised from the maternal tissues and undergo tissue/cell-type differentiation under in vitro conditions. We have developed a method to transform in vitro-grown endosperms using Agrobacterium tumefaciens and standard binary vectors. We show that both aleurone and starchy endosperm cells can be successfully transformed using a short cocultivation with A. tumefaciens cells. The highest transformation rates were obtained with the A. tumefaciens EHA101 strain and the pTF101.1 binary vector. The percentage of aleurone cells transformed following this method varied between 10% and 22% whereas up to the eighth layer of starchy endosperm cells underneath the aleurone layer showed transformed cells. Cultured endosperms undergo normal cell type (aleurone and starchy endosperm) differentiation and storage protein accumulation, making them suitable for cell biology and biochemical studies. In addition, transgenic cultured endosperms are able to express and accumulate epitope-tagged storage proteins that can be isolated for biochemical assays or used for immunolabeling techniques.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.