ArticleMolecular biology of the cell2010
A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
97 citing papers in PubMed, 167 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Article
- Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena.Science China. Life sciences · 2026Article
- Host-transposable element coexistence: a matter of resistance, tolerance and trade-off.The EMBO journal · 2026Review
- Soma-derived 30-nt small RNAs are coupled with chromosome breakage and precisely target nontransposon DNA against elimination inScience advances · 2025Article
- How and when organisms edit their own genomes.Nature genetics · 2025Review
- Relaxed DNA substrate specificity of transposases involved in programmed genome rearrangement.Nucleic acids research · 2025Article
- Nuclear dualism without extensive DNA elimination in the ciliateProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia.Journal of cell science · 2024Article
- Application of RNA interference and protein localization to investigate housekeeping and developmentally regulated genes in the emerging model protozoan Paramecium caudatum.Communications biology · 2024Article
- From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists.Marine life science & technology · 2024Article
- A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination.eLife · 2024Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus.G3 (Bethesda, Md.) · 2023Article
- Origins of genome-editing excisases as illuminated by the somatic genome of the ciliateProceedings of the National Academy of Sciences of the United States of America · 2023Article
- MITE infestation accommodated by genome editing in the germline genome of the ciliateProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A Novel Gene Controls a New Structure: PiggyBac Transposable Element-Derived 1, Unique to Mammals, Controls Mammal-Specific Neuronal Paraspeckles.Molecular biology and evolution · 2022Article
- Epigenetic influences of mobile genetic elements on ciliate genome architecture and evolution.The Journal of eukaryotic microbiology · 2022Review
- Structural insights into the evolution of the RAG recombinase.Nature reviews. Immunology · 2022Review
- Review
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Transposons comprise large fractions of eukaryotic genomes and provide genetic reservoirs for the evolution of new cellular functions. We identified TPB2, a homolog of the piggyBac transposase gene that is required for programmed DNA deletion in Tetrahymena. TPB2 was expressed exclusively during the time of DNA excision, and its encoded protein Tpb2p was localized in DNA elimination heterochromatin structures. Notably, silencing of TPB2 by RNAi disrupts the final assembly of these heterochromatin structures and prevents DNA deletion to occur. In vitro studies revealed that Tpb2p is an endonuclease that produces double-strand breaks with four-base 5' protruding ends, similar to the ends generated during DNA deletion. These findings suggest that Tpb2p plays a key role in the assembly of specialized DNA elimination chromatin architectures and is likely responsible for the DNA cleavage step of programmed DNA deletion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.