ArticleJournal of the Royal Society, Interface2010
Emergence of a prestressed eukaryotic nucleus during cellular differentiation and development.
Article in Journal of the Royal Society, Interface, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Peripheral heterochromatin tethering is required for chromatin-based nuclear mechanical response.Nucleic acids research · 2025Article
- Dynamic Stimulations with Bioengineered Extracellular Matrix-Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Article
- Strain-dependent elastography of cancer cells reveals heterogeneity and stiffening due to attachment.Journal of biomechanics · 2023Article
- Ezh2 harnesses the intranuclear actin cytoskeleton to remodel chromatin in differentiating Th cells.iScience · 2021Article
- Aqueous outflow regulation - 21st century concepts.Progress in retinal and eye research · 2021Review
- Microscopy deep learning predicts virus infections and reveals mechanics of lytic-infected cells.iScience · 2021Article
- Article
- Regulation and Physiological Significance of the Nuclear Shape in Plants.Frontiers in plant science · 2021Review
- The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.Biophysical journal · 2020Article
- ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration.Nature communications · 2020Article
- Effect of Nuclear Stiffness on Cell Mechanics and Migration of Human Breast Cancer Cells.Frontiers in cell and developmental biology · 2020Article
- Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransduction.Scientific reports · 2019Article
- Article
- Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development.Nature communications · 2018Article
- Compressive force induces reversible chromatin condensation and cell geometry-dependent transcriptional response.Molecular biology of the cell · 2018Article
- Tissue engineering the cancer microenvironment-challenges and opportunities.Biophysical reviews · 2018Review
- Nuclear mechanosensing.Emerging topics in life sciences · 2018Article
- Human mesenchymal stem cell basal membrane bending on gratings is dependent on both grating width and curvature.Scientific reports · 2018Article
- Regulation of genome organization and gene expression by nuclear mechanotransduction.Nature reviews. Molecular cell biology · 2017Review
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2 authors.
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Abstract
Nuclear shape and size are emerging as mechanistic regulators of genome function. Yet, the coupling between chromatin assembly and various nuclear and cytoplasmic scaffolds is poorly understood. The present work explores the structural organization of a prestressed nucleus in a variety of cellular systems ranging from cells in culture to those in an organism. A combination of laser ablation and cellular perturbations was used to decipher the dynamic nature of the nucleo-cytoplasmic contacts. In primary mouse embryonic fibroblasts, ablation of heterochromatin nodes caused an anisotropic shrinkage of the nucleus. Depolymerization of actin and microtubules, and inhibition of myosin motors, resulted in the differential stresses that these cytoplasmic systems exert on the nucleus. The onset of nuclear prestress was then mapped in two contexts--first, in the differentiation of embryonic stem cells, where signatures of prestress appeared with differentiation; second, at an organism level, where nuclear or cytoplasmic laser ablations of cells in the early Drosophila embryo induced a collapse of the nucleus only after cellularization. We thus show that the interplay of physical connections bridging the nucleus with the cytoplasm governs the size and shape of a prestressed eukaryotic nucleus.
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