ArticleJournal of biotechnology2010
Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: a way for producing functional protein in prokaryotic microorganism.
Article in Journal of biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- The Effect of Protein FAM172A on Proliferation in HepG2 Cells and Investigation of the Possible Molecular Mechanism.Analytical cellular pathology (Amsterdam) · 2019Article
- Prokaryotic Expression ofBioMed research international · 2017Article
- A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB.BMC veterinary research · 2015Article
- Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.Vaccines · 2013Article
- Transmissible gastroenteritis virus: identification of M protein-binding peptide ligands with antiviral and diagnostic potential.Antiviral research · 2013Article
- Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses.Virology journal · 2013Article
- Article
- Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus.Virus genes · 2011Article
- Characterization and utility of monoclonal antibodies against spike protein of transmissible gastroenteritis virus.Letters in applied microbiology · 2011Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
- Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro.Virology · 2011Article
- Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection.Biotechnology letters · 2011Article
- Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.Journal of biotechnology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on the bioinformatics analysis of the gene encoding glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) isolate HH08, two gene fragments were amplified by polymerase chain reaction (PCR), deleting the signal peptide and transmembrane sequences in GP5 gene. Both gene fragments were designated GP5a and GP5b, respectively. They were ligated with a linker and cloned into prokaryotic expression vector, pET-30a. Expression of the protein of interest was induced by isopropyl beta-d-1-thiogalactopyranoside. The purified protein was used as an immunogen to elicit antibody in rabbit. The immunoreactivity of the protein was determined using ELISA and Western blot. Biologically active GP5 and anti-GP5 antibody inhibited cell infection by PRRSV. Moreover, the antibody produced in this study was capable of detecting the cell infection by PRRSV and distinguishing this virus from other viruses.
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