ArticleStem cell reviews and reports2010
Hyperpolarization induces differentiation in human cardiomyocyte progenitor cells.
Article in Stem cell reviews and reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Information integration during bioelectric regulation of morphogenesis of the embryonic frog brain.iScience · 2023Article
- Activation of Kir2.1 improves myocardial fibrosis by inhibiting CaActa biochimica et biophysica Sinica · 2023Article
- The multifaceted nature of endogenous cardiac regeneration.Frontiers in cardiovascular medicine · 2023Review
- Electrostatic polarization fields trigger glioblastoma stem cell differentiation.Nanoscale horizons · 2022Article
- Effects of Ruanmailing in Blocking Early Stages of Atherosclerosis by TNF-Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Quantum Tunneling-Induced Membrane Depolarization Can Explain the Cellular Effects Mediated by Lithium: Mathematical Modeling and Hypothesis.Membranes · 2021Article
- Tunable electroconductive decellularized extracellular matrix hydrogels for engineering human cardiac microphysiological systems.Biomaterials · 2021Article
- Oscillating shear stress mediates mesenchymal transdifferentiation of EPCs by the Kir2.1 channel.Heart and vessels · 2020Article
- Polypyrrole-Incorporated Conducting Constructs for Tissue Engineering Applications: A Review.Bioelectricity · 2020Review
- Membrane Potential Depolarization Alters Calcium Flux and Phosphate Signaling During Osteogenic Differentiation of Human Mesenchymal Stem Cells.Bioelectricity · 2019Article
- Conductive Silk-Polypyrrole Composite Scaffolds with Bioinspired Nanotopographic Cues for Cardiac Tissue Engineering.Journal of materials chemistry. B · 2018Article
- Bioelectric signaling in regeneration: Mechanisms of ionic controls of growth and form.Developmental biology · 2018Review
- Cardiac Progenitor Cells and the Interplay with Their Microenvironment.Stem cells international · 2017Review
- Bioelectric signalling via potassium channels: a mechanism for craniofacial dysmorphogenesis in KCNJ2-associated Andersen-Tawil Syndrome.The Journal of physiology · 2016Article
- Endogenous gradients of resting potential instructively pattern embryonic neural tissue via Notch signaling and regulation of proliferation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Stem Cells and Progenitor Cells for Tissue-Engineered Solutions to Congenital Heart Defects.Biomarker insights · 2015Review
- Local and long-range endogenous resting potential gradients antagonistically regulate apoptosis and proliferation in the embryonic CNS.The International journal of developmental biology · 2015Article
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the past years, cardiovascular progenitor cells have been isolated from the human heart and characterized. These cells can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells and are therefore of great value for investigation of the mechanisms that drive progenitor cell function and plasticity, drug testing and, potentially, therapeutical purposes. In this respect, most studies have focused on enhancing differentiation with chemicals or growth factors, or co-culture with other cell types. Although they have revealed important mechanisms, protocols need to be established that exclude the need for such factors when one considers using progenitor cells to repair the human heart. In this study we tested whether we could induce cardiomyogenic differentiation of human cardiomyocyte progenitor cells (CMPCs) by altering their membrane potential. We induced hyperpolarization in CMPCs by either co-culturing them with a K(ir)2.1-overexpressing cell line or by overnight culture in medium containing low potassium concentrations. Hyperpolarization led to increased intracellular calcium concentrations, activation of calcineurin signaling, increased cardiac-specific gene and protein expression levels and, ultimately, to the formation of spontaneously beating cardiomyocytes. Thus, hyperpolarization is sufficient to induce differentiation of CMPCs, thereby revealing a novel mechanism for cardiomyogenic differentiation of heart-derived progenitor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.