ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2010
Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Birth of offspring derived from cryopreserved rat sperm after shipment in a Styrofoam box at -80°C.Experimental animals · 2025Article
- Paternal age impairs in vitro embryo and in vivo fetal development in murine.Scientific reports · 2022Article
- Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats.Scientific reports · 2020Article
- Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa.Reproductive biology and endocrinology : RB&E · 2016Article
- Short-term storage of rat sperm in the presence of various extenders.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Extracellular ATP and dibutyryl cAMP enhance the freezability of rat epididymal sperm.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
We evaluated the cryosurvival of rat epididymal sperm preserved in raffinose-modified Krebs-Ringer bicarbonate-egg yolk extender supplemented with various energy-yielding substrates (glucose, pyruvate, lactate, and ATP) and assessed the effect on sperm oxygen consumption. The incubation of sperm at 37 degrees C for 10 min in lactate-free extender decreased sperm motility and oxygen consumption before and after thawing compared with those of sperm in glucose- and pyruvate-free mediums. We then focused on the effect of supplementing the extender with lactate (0, 10.79, 21.58, 32.37, and 43.16 mM) and found that sperm frozen and thawed in extender supplemented with 32.37 mM lactate exhibited the highest motility. When we supplemented extender containing 32.37 mM lactate with ATP (0, 0.92, 1.85, 3.70, and 5.55 mM), sperm frozen and thawed in the extender supplemented with 1.85 mM ATP exhibited considerably higher motility and viability than those of sperm frozen and thawed in ATP-free extender. These results provide the first evidence that supplementation of the raffinose-modified Krebs-Ringer bicarbonate-egg yolk extender with 32.37 mM lactate and 1.85 mM ATP increases of number of motile sperm before freezing and enhances the cryosurvival of rat sperm. These supplements to the extender may enhance sperm cryosurvival by improving the metabolic capacity of sperm before freezing.
Indexed as
Identifiers
20353689PMC2846002What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.