ArticleStem cell reviews and reports2010
Endoplasmic reticulum stress signals in defined human embryonic stem cell lines and culture conditions.
Article in Stem cell reviews and reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Nitrogen-doped carbon quantum dot regulates cell proliferation and differentiation by endoplasmic reticulum stress.Animal cells and systems · 2024Article
- Nonsense-mediated mRNA decay: a 'nonsense' pathway makes sense in stem cell biology.Nucleic acids research · 2018Review
- New insights into the unfolded protein response in stem cells.Oncotarget · 2016Review
- Stress signaling in mammalian oocytes and embryos: a basis for intervention and improvement of outcomes.Cell and tissue research · 2016Review
- Blocking autocrine VEGF signaling by sunitinib, an anti-cancer drug, promotes embryonic stem cell self-renewal and somatic cell reprogramming.Cell research · 2014Article
- Automated method for the rapid and precise estimation of adherent cell culture characteristics from phase contrast microscopy images.Biotechnology and bioengineering · 2014Article
- Mechanical stress stimulates the osteo/odontoblastic differentiation of human stem cells from apical papilla via erk 1/2 and JNK MAPK pathways.BioMed research international · 2014Article
- Molecular characteristics of cancer stem-like cells derived from human breast cancer cells.Anticancer research · 2013Article
- Influence of Iron Oxide Nanoparticles on Innate and Genetically Modified Secretion Profiles of Mesenchymal Stem Cells.IEEE transactions on magnetics · 2013Article
- Article
- Mechanical strain downregulates C/EBPβ in MSC and decreases endoplasmic reticulum stress.PloS one · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Human embryonic stem cells (hESCs) are especially resistant to several cellular stresses, but the existence and induction of Endoplasmic Reticulum (ER) stress by culture conditions are unknown. Using qPCR, here, we investigated the behavior of the principal sensors of ER stress and their relation with the feeder layer, the type of conditioned media used in feeder free systems and the upregulation of several differentiation markers. We observed the preservation of pluripotency, and detected differential expression of differentiation markers in HS181 and SHEF1 hESCs growing on Adipose-derived mesenchymal stem cells (ASCs) and feeder-free system with different conditioned media (HEF-CM and ASC-CM). Taken together, these results demonstrate evidence of ER stress events that cells must resolve to survive and maintenance of markers of pluripotency. The early differentiation status defined could progress into a more differentiated state, and may be influenced by culture conditions.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.