ArticleCellular and molecular life sciences : CMLS2010
Transcription factor-based modulation of neural stem cell differentiation using direct protein transduction.
Article in Cellular and molecular life sciences : CMLS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering.Journal of Zhejiang University. Science. B · 2022Article
- Protein Production and Purification of a Codon-Optimized Human NGN3 Transcription Factor from E. coli.The protein journal · 2021Article
- Article
- Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor.Applied microbiology and biotechnology · 2021Article
- Codon Optimization, Cloning, Expression, Purification, and Secondary Structure Determination of Human ETS2 Transcription Factor.Molecular biotechnology · 2020Article
- Take the shortcut - direct conversion of somatic cells into induced neural stem cells and their biomedical applications.FEBS letters · 2019Review
- Enhanced effect of nuclear localization signal peptide during ultrasound‑targeted microbubble destruction‑mediated gene transfection.Molecular medicine reports · 2017Article
- Cellular Reprogramming Using Protein and Cell-Penetrating Peptides.International journal of molecular sciences · 2017Review
- Optic Nerve Degeneration after Retinal Ischemia/Reperfusion in a Rodent Model.Frontiers in cellular neuroscience · 2017Article
- Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells.International journal of molecular sciences · 2015Review
- Targeted intracellular delivery of proteins with spatial and temporal control.Molecular pharmaceutics · 2015Article
- Efficient differentiation of human embryonic stem cells toward dopaminergic neurons using recombinant LMX1A factor.Molecular biotechnology · 2015Article
- RETRACTED: ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells.PloS one · 2013Article
- Non-genetic modulation of Notch activity by artificial delivery of Notch intracellular domain into neural stem cells.Stem cell reviews and reports · 2012Article
- Cellular reprogramming employing recombinant sox2 protein.Stem cells international · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to conventional gene transfer strategies, the direct introduction of recombinant proteins into cells bypasses the risk of insertional mutagenesis and offers an alternative to genetic intervention. Here, we explore whether protein transduction of the gliogenic transcription factor Nkx2.2 can be used to promote oligodendroglial differentiation of mouse embryonic stem cell (ESC)-derived neural stem cells (NSC). To that end, a recombinant cell-permeant form of Nkx2.2 protein was generated. Exposure of ESC-derived NSC to the recombinant protein and initiation of differentiation resulted in a two-fold increase in the number of oligodendrocytes. Furthermore, Nkx2.2-transduced cells exhibited a more mature oligodendroglial phenotype. Comparative viral gene transfer studies showed that the biological effect of Nkx2.2 protein transduction is comparable to that obtained by lentiviral transduction. The results of this proof-of-concept study depict direct intracellular delivery of transcription factors as alternative modality to control lineage differentiation in NSC cultures without genetic modification.
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Registered trials
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