ArticleTransgenic research2011
Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Genetic and Molecular Quality Control of Genetically Engineered Mice.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Development of a porcine model of phenylketonuria with a humanized R408W mutation for gene editing.PloS one · 2021Article
- Changes in Skeletal Muscle and Body Weight on Sleeping Beauty Transposon-Mediated Transgenic Mice Overexpressing Pig mIGF-1.Biochemical genetics · 2018Article
- Cyclooxygenase 2: protein-protein interactions and posttranslational modifications.Physiological genomics · 2017Review
- Genome editing and genetic engineering in livestock for advancing agricultural and biomedical applications.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Rat Genome and Model Resources.ILAR journal · 2017Review
- Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes.PloS one · 2017Article
- Novel strategies for gene trapping and insertional mutagenesis mediated byMobile genetic elements · 2013Article
- Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT).Transgenic research · 2013Article
- Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Completion of the swine genome will simplify the production of swine as a large animal biomedical model.BMC medical genomics · 2012Article
- Efficient TALEN-mediated gene knockout in livestock.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Precision editing of large animal genomes.Advances in genetics · 2012Review
- Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis.Mobile DNA · 2011Article
- Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system.Transgenic research · 2011Article
- Narrowing a region on rat chromosome 13 that protects against hypertension in Dahl SS-13BN congenic strains.American journal of physiology. Heart and circulatory physiology · 2011Article
- Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa.PloS one · 2011Article
- Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome.PloS one · 2011Article
- Gene targeting in the rat: advances and opportunities.Trends in genetics : TIG · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Heightened interest in relevant models for human disease increases the need for improved methods for germline transgenesis. We describe a significant improvement in the creation of transgenic laboratory mice and rats by chemical modification of Sleeping Beauty transposons. Germline transgenesis in mice and rats was significantly enhanced by in vitro cytosine-phosphodiester-guanine methylation of transposons prior to injection. Heritability of transgene alleles was also greater from founder mice generated with methylated versus non-methylated transposon. The artificial methylation was reprogrammed in the early embryo, leading to founders that express the transgenes. We also noted differences in transgene insertion number and structure (single-insert versus concatemer) based on the influence of methylation and plasmid conformation (linear versus supercoiled), with supercoiled substrate resulting in efficient transpositional transgenesis (TnT) with near elimination of concatemer insertion. Combined, these substrate modifications resulted in increases in both the frequency of transgenic founders and the number of transgenes per founder, significantly elevating the number of potential transgenic lines. Given its simplicity, versatility and high efficiency, TnT with enhanced Sleeping Beauty components represents a compelling non-viral approach to modifying the mammalian germline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.