ArticlePloS one2010
Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Improved metabolic health alters host metabolism in parallel with changes in systemic xeno-metabolites of gut origin.PloS one · 2014Trial
- Fasting requires Peroxiredoxin 2 and Peroxiredoxin 6 to coordinate redox dependent mitochondrial and lipid remodelling in Caenorhabditis elegans.Redox biology · 2026Article
- Chronic exercise improves hepatic acylcarnitine handling.iScience · 2024Article
- Exercise plasma metabolomics and xenometabolomics in obese, sedentary, insulin-resistant women: impact of a fitness and weight loss intervention.American journal of physiology. Endocrinology and metabolism · 2019Article
- Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans.American journal of physiology. Endocrinology and metabolism · 2019Article
- A novel amino acid and metabolomics signature in mice overexpressing muscle uncoupling protein 3.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Unraveling biochemical pathways affected by mitochondrial dysfunctions using metabolomic approaches.Metabolites · 2014Review
- Fatty acid transport protein 1 (FATP1) localizes in mitochondria in mouse skeletal muscle and regulates lipid and ketone body disposal.PloS one · 2014Article
- Muscle uncoupling protein 3 overexpression mimics endurance training and reduces circulating biomarkers of incomplete β-oxidation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Targeting proteomics to investigate metastasis-associated mitochondrial proteins.Journal of bioenergetics and biomembranes · 2012Review
- Role of intramyocelluar lipids in human health.Trends in endocrinology and metabolism: TEM · 2012Review
- MetaMapp: mapping and visualizing metabolomic data by integrating information from biochemical pathways and chemical and mass spectral similarity.BMC bioinformatics · 2012Article
- Bioinformatics Tools for Mass Spectroscopy-Based Metabolomic Data Processing and Analysis.Current bioinformatics · 2012Article
- Plasma metabolomic profiles reflective of glucose homeostasis in non-diabetic and type 2 diabetic obese African-American women.PloS one · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND/
aimIncomplete or limited long-chain fatty acid (LCFA) combustion in skeletal muscle has been associated with insulin resistance. Signals that are responsive to shifts in LCFA beta-oxidation rate or degree of intramitochondrial catabolism are hypothesized to regulate second messenger systems downstream of the insulin receptor. Recent evidence supports a causal link between mitochondrial LCFA combustion in skeletal muscle and insulin resistance. We have used unbiased metabolite profiling of mouse muscle mitochondria with the aim of identifying candidate metabolites within or effluxed from mitochondria and that are shifted with LCFA combustion rate. METHODOLOGY/PRINCIPAL
findingsLarge-scale unbiased metabolomics analysis was performed using GC/TOF-MS on buffer and mitochondrial matrix fractions obtained prior to and after 20 min of palmitate catabolism (n = 7 mice/condition). Three palmitate concentrations (2, 9 and 19 microM; corresponding to low, intermediate and high oxidation rates) and 9 microM palmitate plus tricarboxylic acid (TCA) cycle and electron transport chain inhibitors were each tested and compared to zero palmitate control incubations. Paired comparisons of the 0 and 20 min samples were made by Student's t-test. False discovery rate were estimated and Type I error rates assigned. Major metabolite groups were organic acids, amines and amino acids, free fatty acids and sugar phosphates. Palmitate oxidation was associated with unique profiles of metabolites, a subset of which correlated to palmitate oxidation rate. In particular, palmitate oxidation rate was associated with distinct changes in the levels of TCA cycle intermediates within and effluxed from mitochondria. CONCLUSIONS/SIGNIFICANCE: This proof-of-principle study establishes that large-scale metabolomics methods can be applied to organelle-level models to discover metabolite patterns reflective of LCFA combustion, which may lead to identification of molecules linking muscle fat metabolism and insulin signaling. Our results suggest that future studies should focus on the fate of effluxed TCA cycle intermediates and on mechanisms ensuring their replenishment during LCFA metabolism in skeletal muscle.
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Registered trials
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