ArticleJournal of clinical microbiology2010
Development of a DNA microarray for detection and serotyping of enterotoxigenic Escherichia coli.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Advances in diagnosis of diseases causing diarrhea in newborn calves.Veterinary research communications · 2025Review
- Genetic diversity of the O antigens of Proteus species and the development of a suspension array for molecular serotyping.PloS one · 2017Article
- Genetic Analysis and Detection of fliC H1 and fliC H12 Genes Coding for Serologically Closely Related Flagellar Antigens in Human and Animal Pathogenic Escherichia coli.Frontiers in microbiology · 2016Article
- The serum resistome of a globally disseminated multidrug resistant uropathogenic Escherichia coli clone.PLoS genetics · 2013Article
- DNA microarrays for the diagnosis of infectious diseases.Medecine et maladies infectieuses · 2012Review
- Molecular and nanotechnologic approaches to etiologic diagnosis of infectious syndromes.Molecular diagnosis & therapy · 2011Review
- An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents.PloS one · 2011Article
- Molecular serotyping of diarrheagenicGut microbesArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterotoxigenic Escherichia coli (ETEC) is a common pathogen worldwide causing infectious diarrhea, especially traveler's diarrhea. Traditional physiological assays, immunoassays, and PCR-based methods for the detection of ETEC target the heat-labile enterotoxin and/or the heat-stable enterotoxin. Separate serotyping methods using antisera are required to determine the ETEC serogroup. In this study, we developed a DNA microarray that can simultaneously detect enterotoxin genes and the 19 most common O serogroup genes in ETEC strains. The specificity and reproducibility of this approach were verified by hybridization to 223 strains: 50 target reference or clinical strains and 173 other strains, including those belonging to other E. coli O serogroups and closely related species. The sensitivity of detection was determined to be 50 ng of genomic DNA or 10(8) CFU per ml of organisms in pure culture. The random PCR strategy used in this study with minimal bias provides an effective alternative to multiplex PCR for the detection of pathogens using DNA microarrays. The assay holds promise for applications in the clinical diagnosis and epidemiological surveillance of pathogenic microorganisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.